102212-99-7 Usage
Description
5-Bromo-2'-deoxyuridine 5'-triphosphate sodium salt (5-BrdUTP) is a synthetic nucleotide analog that is structurally similar to thymidine 5'-triphosphate (dTTP). It is used for incorporation into DNA for studying normal and tumor cell proliferation profiles. 5-BrdUTP can be used as a labeling substrate for terminal deoxynucleotidyl transferase (TdT) reactions or for DNA synthesis. It is also an effective mutagenic agent, and its incorporation into DNA can be detected by various methods, including immunological techniques, biotinylation, or fluorogenic and chromogenic methods.
Uses
Used in Molecular Biology Research:
5-Bromo-2'-deoxyuridine 5'-triphosphate sodium salt is used as a nucleotide analog for incorporation into DNA, allowing researchers to study normal and tumor cell proliferation profiles. This helps in understanding the mechanisms of cell growth and identifying potential therapeutic targets for cancer treatment.
Used in TdT Reactions:
5-BrdUTP serves as a labeling substrate for terminal deoxynucleotidyl transferase (TdT) reactions, which are essential for studying the addition of nucleotides to the 3' ends of DNA strands. This process is crucial for various biological functions, including V(D)J recombination and telomere maintenance.
Used as a Mutagenic Agent:
5-Bromo-2'-deoxyuridine 5'-triphosphate sodium salt is used as an effective mutagenic agent, enabling researchers to introduce mutations into DNA sequences. This can be useful for studying the effects of specific mutations on gene function and regulation.
Used in Detection Techniques:
The incorporation of 5-BrdUTP into DNA can be detected using various methods, such as immunological techniques, biotinylation, or fluorogenic and chromogenic methods. These detection techniques are valuable for monitoring the incorporation of the nucleotide analog and assessing its effects on DNA structure and function.
Used in Drug Development:
5-Bromo-2'-deoxyuridine 5'-triphosphate sodium salt can be employed in the development of drugs targeting DNA synthesis and repair pathways. By studying the effects of 5-BrdUTP on these processes, researchers can identify potential drug candidates that modulate DNA synthesis and repair, which may have therapeutic applications in various diseases, including cancer.
Check Digit Verification of cas no
The CAS Registry Mumber 102212-99-7 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,0,2,2,1 and 2 respectively; the second part has 2 digits, 9 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 102212-99:
(8*1)+(7*0)+(6*2)+(5*2)+(4*1)+(3*2)+(2*9)+(1*9)=67
67 % 10 = 7
So 102212-99-7 is a valid CAS Registry Number.