102783-51-7

Basic information

• Product Name: 2'-Deoxycytidine-5'-triphosphoric acid disodium salt
• Synonyms: dCTP, 100mM Solution;2'-Deoxycytidine-5'-triphospate, trisodium salt;2'-Deoxycytidine-5'-triphosphate(dCTP),trisodium salt,dihydrate;2'-DEOXY-CYTIDINE-5'-TRIPHOSPHATE TETRASODIUM SALT, SOLUTION 100 mM, MOLECULAR BIOLOGY GR;2′-Deoxycytidine 5′-triphosphate disodium salt,dCTP-Na2;2'-Deoxycytidine-5'-triphosphate, 100MM Solution;dCTP,4Na;2'-DeoxyCTP DisodiuM
• CAS NO: 102783-51-7
• Molecular Formula: C9H14N3Na2O13P3
• Molecular Weight: 511.12
• EINECS: 2017-001-1
• Product Categories: Bases & Related Reagents;Carbohydrates & Derivatives;Heterocycles;Nucleotides;Phosphorylating and Phosphitylating Agents;nucleoside
• Mol File: 102783-51-7.mol
• Article Data: 0

Chemical Properties

• Melting Point: >0°C
• Appearance: colorless/aqueous solution
• Storage Temp.: −20°C
• Solubility: Water (Slightly)
• Water Solubility: Soluble in water.
• Stability: Hygroscopic
• CAS DataBase Reference: 2'-Deoxycytidine-5'-triphosphoric acid disodium salt(CAS DataBase Reference)
• NIST Chemistry Reference: 2'-Deoxycytidine-5'-triphosphoric acid disodium salt(102783-51-7)
• EPA Substance Registry System: 2'-Deoxycytidine-5'-triphosphoric acid disodium salt(102783-51-7)

Safety Data

• Hazard Codes: Xn,Xi
• Statements: 23/24/25-36/37/38-39/23/24/25-20/21/22-39
• Safety Statements: 26-36-45-37/39-22-20/21-1/2
• WGK Germany: 3
• F: 10-21
• Hazardous Substances Data: 102783-51-7(Hazardous Substances Data)

Usage

Description

2'-Deoxycytidine-5'-triphosphoric acid disodium salt, also known as dCTP, is a nucleoside triphosphate that serves as a crucial metabolite in the synthesis of DNA. It is a white powder with a clear colorless solution at a concentration of 100 mM and a pH of 8.3. dCTP is specially manufactured and purified to achieve the highest possible chemical purity, containing at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

Uses

Used in Molecular Biology and DNA Synthesis:
2'-Deoxycytidine-5'-triphosphoric acid disodium salt is used as a substrate for DNA synthesis with DNA polymerases and reverse transcriptases. It plays a vital role in various molecular biology methods, including polymerase chain reaction (PCR), DNA sequencing, and other DNA polymerase-driven reactions.
Used in PCR Applications:
In the polymerase chain reaction industry, 2'-Deoxycytidine-5'-triphosphoric acid disodium salt is used as a key component for amplifying specific DNA sequences. It is essential for the PCR process, as it provides the necessary energy and building blocks for the synthesis of new DNA strands.
Used in DNA Sequencing:
In the field of DNA sequencing, 2'-Deoxycytidine-5'-triphosphoric acid disodium salt is utilized as a nucleotide precursor for the incorporation of dCTP into the growing DNA strand. This allows for the determination of the order of nucleotides in a DNA molecule, which is crucial for understanding genetic information and identifying genetic variations.
Used in Research and Diagnostics:
2'-Deoxycytidine-5'-triphosphoric acid disodium salt is also used in research and diagnostic applications, where it aids in the study of DNA replication, repair, and other cellular processes. It is an essential tool for scientists and researchers working in the fields of genetics, molecular biology, and biotechnology.

Biochem/physiol Actions

2′-Deoxycytidine 5′-triphosphate (dCTP) is used to synthesize DNA by DNA polymerases or reverse transcriptases. dCTP is an allosteric regulator of deoxycytidylate (dCMP) deaminase. It is used in studies of nucleotide pools, nucleotide selectivity and enzyme specificity studies, such as the preferred incorporation of dCTP by Rev1, a γ-family DNA polymerase.