104582-29-8 Usage
Description
3-[2-N-(BIOTINYL)AMINOETHYLDITHIO]PROPANOIC ACID, also known as Biotin-SS-COOH, is a cleavable, biotinylated crosslinker used in various chemical biology applications. It is a white powder that possesses dual functionality, including enrichment through the biotin component and an embedded disulfide bridge for cleavage under reducing conditions. This unique structure makes it a versatile tool for labeling target proteins in biological experiments and assays.
Uses
Used in Chemical Biology:
3-[2-N-(BIOTINYL)AMINOETHYLDITHIO]PROPANOIC ACID is used as a crosslinker for the synthesis of chemical biology tools. It is particularly useful for labeling target proteins in biological experiments and assays due to its dual functionality.
Used in Enrichment Techniques:
In the field of proteomics and biochemistry, 3-[2-N-(BIOTINYL)AMINOETHYLDITHIO]PROPANOIC ACID is used as an enrichment agent for the isolation and purification of specific proteins. The biotin component allows for easy detection and capture of target proteins, making it a valuable tool in protein analysis.
Used in Cleavage Studies:
3-[2-N-(BIOTINYL)AMINOETHYLDITHIO]PROPANOIC ACID is used as a cleavable linker in the study of protein-protein interactions and other biological processes involving disulfide bonds. The embedded disulfide bridge can be cleaved under reducing conditions, providing insights into the structure and function of proteins.
Used in Pharmaceutical Research:
In the pharmaceutical industry, 3-[2-N-(BIOTINYL)AMINOETHYLDITHIO]PROPANOIC ACID is used as a key component in the development of novel drug delivery systems and targeted therapies. Its ability to label and enrich specific proteins makes it a valuable tool for drug discovery and development.
Used in Diagnostic Applications:
3-[2-N-(BIOTINYL)AMINOETHYLDITHIO]PROPANOIC ACID is used as a diagnostic agent in the development of new tests and assays for the detection and monitoring of various diseases. Its dual functionality allows for the specific targeting and detection of biomarkers, making it a promising tool in the field of diagnostics.
Check Digit Verification of cas no
The CAS Registry Mumber 104582-29-8 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,0,4,5,8 and 2 respectively; the second part has 2 digits, 2 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 104582-29:
(8*1)+(7*0)+(6*4)+(5*5)+(4*8)+(3*2)+(2*2)+(1*9)=108
108 % 10 = 8
So 104582-29-8 is a valid CAS Registry Number.
InChI:InChI=1/C15H25N3O4S3/c19-12(16-6-8-25-24-7-5-13(20)21)4-2-1-3-11-14-10(9-23-11)17-15(22)18-14/h10-11,14H,1-9H2,(H,16,19)(H,20,21)(H2,17,18,22)/t10-,11-,14-/m0/s1
104582-29-8Relevant articles and documents
Design and synthesis of a bifunctional label for selection of β-lactamase displayed on filamentous bacteriophage by catalytic activity
Marchand-Brynaert, Jacqueline,Bouchet, Michele,Touillaux, Roland,Beauve, Cecile,Fastrez, Jacques
, p. 5591 - 5606 (2007/10/03)
A bifunctional activity label 1c has been constructed for the selection of active β-lactamases displayed on filamentous bacteriophage. It features an original 6-sulfonylamido-penam sulfone moiety, as β-lactamase suicide-inhibitor, and a biotinyl residue, for separation by affinity chromatography, connected through a linker including a cleavable disulfide bond. The inhibitor 28 resulted from coupling of methoxymethyl 6-aminopenicillinate 8 with N-protected (aminoethoxy)ethoxyethanesulfonyl chloride 23, followed by oxidation into the corresponding sulfone 25, and usual deprotections. The biotinyl ester 32 reacted with 3-(2-aminoethyldithio)propanoic acid 31 as linker, to give 33 which was further activated as pentafluorophenol ester 34b. Final coupling of the building blocks 28 and 34b gave the target label 1c.
