108321-84-2 Usage
Description
H-PHE-AMC TFA, also known as L-Phenylalanine 7-Amido-4-methylcoumarin, Trifluoroacetate Salt, is a fluorogenic substrate specifically designed for the detection and measurement of phenylalanine peptidase activity. This white powder compound serves as a valuable tool in biochemical research and assays, providing a blue fluorescent signal upon enzymatic cleavage, which allows for the quantification and analysis of enzymatic activity.
Uses
Used in Biochemical Research:
H-PHE-AMC TFA is used as a fluorogenic substrate for the detection and measurement of phenylalanine peptidase activity. The blue fluorescent signal generated upon enzymatic cleavage aids researchers in understanding the kinetics and characteristics of the enzyme, as well as its role in various biological processes.
Used in Enzyme Assays:
In enzyme assays, H-PHE-AMC TFA is used as a reactant/reagent to monitor the activity of phenylalanine peptidase. The blue fluorescence emitted upon substrate cleavage allows for the quantification of enzymatic activity, which is crucial for evaluating enzyme function and potential interactions with other molecules or inhibitors.
Used in Immunology:
H-PHE-AMC TFA is used as a reactant/reagent for the activation of lymphocyte progranzyme by dipeptidyl peptidase I. This application is particularly relevant in the study of immune cell function and the role of proteolytic enzymes in modulating immune responses.
Used in Pharmaceutical Development:
The ability of H-PHE-AMC TFA to generate a fluorescent signal upon enzymatic cleavage makes it a valuable tool in the development of new drugs targeting phenylalanine peptidase. By monitoring the enzyme's activity in the presence of potential drug candidates, researchers can assess the efficacy and selectivity of these compounds in inhibiting or modulating the enzyme's function.
Used in Diagnostic Applications:
H-PHE-AMC TFA can be employed in diagnostic assays to detect and quantify phenylalanine peptidase activity in biological samples. This information can be useful in diagnosing certain conditions or monitoring the progression of diseases where phenylalanine peptidase activity may be implicated.
Check Digit Verification of cas no
The CAS Registry Mumber 108321-84-2 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,0,8,3,2 and 1 respectively; the second part has 2 digits, 8 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 108321-84:
(8*1)+(7*0)+(6*8)+(5*3)+(4*2)+(3*1)+(2*8)+(1*4)=102
102 % 10 = 2
So 108321-84-2 is a valid CAS Registry Number.
InChI:InChI=1/C19H18N2O3.C2HF3O2/c1-12-9-18(22)24-17-11-14(7-8-15(12)17)21-19(23)16(20)10-13-5-3-2-4-6-13;3-2(4,5)1(6)7/h2-9,11,16H,10,20H2,1H3,(H,21,23);(H,6,7)/t16-;/m0./s1
