114684-52-5Relevant articles and documents
Kinetically Controlled Peptide Bond Formation in Anhydrous Alcohol Catalyzed by the Industrial Protease Alcalase
Chen, Shui-Tein,Chen, Shiah-Yun,Wang, Kung-Tsung
, p. 6960 - 6965 (2007/10/02)
The industrial alkaline protease alcalase has been found to be very stable (half life > 5 days in ethanol or 2-methyl-2-propanol) and active in alcoholic solvents (except methanol).Procedures have been developed for alcalase-catalyzed, kinetically controlled peptide bond formation in anhydrous alcohol (ethanol, 2-methyl-2-propanol).Studies of the selectivity of an alcalase-catalyzed reaction show that only L-amino acid acyl donors are substrates at the p-1 subsite of alcalase; at the p-1' subsite both D- and L-amino acid nucleophiles are substrates.Other amino compounds such as benzylamine and phenylhydrazine are good nucleophiles.Studies of the effect of the water content of the reaction solution on the yield in the synthesis of Moz-Phe-Leu-NH2 showed that the 95percent yield obtained in anhydrous 2-methyl-2-propanol was decreased to 48percent in 2-methyl-2-propanol containing 4.86percent water.
New methods and reagents in organic synthesis. 69. A new synthesis of alpha-amino acid and peptide amides of aromatic amines using a modified Curtius reaction with diphenyl phosphorazidate.
Shioiri,Murata,Hamada
, p. 2698 - 2704 (2007/10/02)
-
