115926-60-8

Basic information

• Product Name: SPE I
• Synonyms: RESTRICTION ENDONUCLEASES SPE I;SPE I;SPE I RESTRICTION ENZYME;RESTRICTION ENDONUCLEASE SPE I FROM*SPHA EROTILUS SP;spei5'...actagt...3'isolatedfromsphaerotilusspecies;spe i from sphaerotilus sp.;RESTRICTION ENDONUCLEASE SPE I
• CAS NO: 115926-60-8
• Molecular Formula: Unspecified
• Molecular Weight: 0
• Product Categories: 3.1.x.x Acting on esters;3.x.x.x Hydrolases;Molecular Biology;Molecular Biology Enzymes;Molecular Biology Tested;Restriction EndonucleasesApplication Index;Restriction EnzymesEnzyme Class Index
• Mol File: 115926-60-8.mol
• Article Data: 0

Chemical Properties

• Appearance: /buffered aqueous glycerol solution
• Storage Temp.: −20°C
• CAS DataBase Reference: SPE I(CAS DataBase Reference)
• NIST Chemistry Reference: SPE I(115926-60-8)
• EPA Substance Registry System: SPE I(115926-60-8)

Safety Data

• WGK Germany: 3
• Hazardous Substances Data: 115926-60-8(Hazardous Substances Data)

Usage

Description

SPE I, also known as the restriction endonuclease Spe I, is an enzyme that recognizes and cuts specific DNA sequences. It is derived from the bacterium Streptomyces species and is widely used in molecular biology and genetic research. The enzyme has the ability to recognize the sequence A↓CTAGT and generates fragments with 5′-cohesive termini, which are essential for various applications in genetic engineering and molecular cloning.

Uses

Used in Genetic Engineering:
SPE I is used as a restriction enzyme for genetic engineering applications. It is employed to cut DNA at specific recognition sites, allowing for the manipulation and recombination of DNA fragments. This is crucial for the construction of recombinant DNA molecules, gene cloning, and the creation of genetically modified organisms.
Used in Molecular Cloning:
In molecular cloning, SPE I is used as a tool to generate specific DNA fragments with 5′-cohesive termini. These cohesive ends facilitate the ligation of DNA fragments, enabling the insertion of target genes into vectors for cloning and expression in host organisms.
Used in DNA Fingerprinting:
SPE I is utilized as a restriction enzyme in DNA fingerprinting techniques, such as restriction fragment length polymorphism (RFLP) analysis. By cutting DNA at specific sites, it helps to generate a unique pattern of DNA fragments that can be used to identify individuals or strains of organisms.
Used in Genomic Research:
In genomic research, SPE I is employed to analyze and compare the genomic sequences of different organisms. By cutting DNA at specific recognition sites, it allows researchers to study gene organization, gene regulation, and the functional elements within the genome.
Used in Diagnostics:
SPE I is used in the development of diagnostic tools for genetic diseases and conditions. By cutting DNA at specific sites, it can help identify mutations or variations in the DNA sequence that are associated with specific diseases, enabling accurate diagnosis and personalized treatment options.
Used in DNA Sequencing:
In DNA sequencing, SPE I is used to generate specific DNA fragments that can be sequenced and analyzed. This information is crucial for understanding the genetic basis of various traits and diseases, as well as for identifying potential drug targets and developing new therapies.