116919-18-7 Usage
Description
Biotin-maleimide is a biotin-specific thiol-containing reagent used to biotinylate different proteins through artificially induced or natural sulfhydryl groups. It serves as a universal, multipurpose, thiol-specific probe and can be utilized in various applications across different industries.
Uses
Used in Biochemical Research:
Biotin-maleimide is used as a biotinylation agent for proteins, enabling the detection and analysis of deprotected cysteine thiols present on immobilized peptides. This application is crucial for studying protein-protein interactions and understanding the role of thiol groups in biological systems.
Used in Diagnostics and Detection:
In the diagnostics industry, Biotin-maleimide is used as a detection reagent in dot blots to identify protein SH groups. This allows for the assessment of protein expression levels, post-translational modifications, and the presence of specific proteins in biological samples.
Used in Drug Development:
Biotin-maleimide can be employed in the development of drugs targeting proteins with thiol groups. By biotinylating these proteins, researchers can study their structure, function, and interactions with other molecules, leading to the discovery of potential therapeutic agents.
Used in Analytical Techniques:
In the field of analytical chemistry, Biotin-maleimide is used as a thiol-specific probe to analyze and quantify thiol-containing compounds. This aids in understanding the role of these compounds in various chemical reactions and processes.
Overall, Biotin-maleimide is a versatile reagent with applications in biochemical research, diagnostics, drug development, and analytical techniques, making it an essential tool in various scientific and industrial fields.
Check Digit Verification of cas no
The CAS Registry Mumber 116919-18-7 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,1,6,9,1 and 9 respectively; the second part has 2 digits, 1 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 116919-18:
(8*1)+(7*1)+(6*6)+(5*9)+(4*1)+(3*9)+(2*1)+(1*8)=137
137 % 10 = 7
So 116919-18-7 is a valid CAS Registry Number.
InChI:InChI=1/C20H29N5O5S/c26-15(7-2-1-5-11-25-17(28)9-10-18(25)29)23-24-16(27)8-4-3-6-14-19-13(12-31-14)21-20(30)22-19/h9-10,13-14,19H,1-8,11-12H2,(H,23,26)(H,24,27)(H2,21,22,30)/t13-,14-,19-/m0/s1
116919-18-7Relevant articles and documents
Method for preparing biotin maleimide
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Paragraph 0025; 0040; 0045; 0046; 0051, (2019/04/06)
The invention discloses a method for preparing biotin maleimide. The method comprises the following steps of: 1, reacting 6-maleimidohexanoic acid compound (1) with N-hydroxysuccinimide under the action of a condensing agent to obtain a 6-maleimidohexanoic acid N-hydroxysuccinimide ester compound (2); 2, treating the 6-maleimidohexanoic acid N-hydroxysuccinimide ester compound (2) by using hydrazine hydrate to obtain a 6- maleimidocaproyl hydrazide compound (3); 3, reacting a biotin compound (4) with an acyl halide reagent to obtain a biotinyl acyl halide compound (5); and 4, reacting the 6-maleimidocaproyl hydrazide compound (3) with the biotinyl acyl halide compound (5) to obtain a biotin maleimide compound (6). The method for preparing the biotin maleimide, disclosed by the invention, has the beneficial effects of being suitable for scale-up production, easily available in raw materials, simple in preparation process and friendly to environment.
