120999-41-9

Basic information

• Product Name: Benzenebutanenitrile, a-hydroxy-, (R)-
• CAS NO: 120999-41-9
• Molecular Formula: C10H11NO
• Molecular Weight: 161.203
• Mol File: 120999-41-9.mol
• Article Data: 39

Chemical Properties

• CAS DataBase Reference: Benzenebutanenitrile, a-hydroxy-, (R)-(CAS DataBase Reference)
• NIST Chemistry Reference: Benzenebutanenitrile, a-hydroxy-, (R)-(120999-41-9)
• EPA Substance Registry System: Benzenebutanenitrile, a-hydroxy-, (R)-(120999-41-9)

Safety Data

• Hazardous Substances Data: 120999-41-9(Hazardous Substances Data)

Upstream product

• 104-53-0 3-phenyl-propionaldehyde 
• 7677-24-9 trimethylsilyl cyanide 
• 108-05-4 vinyl acetate 
• 121054-03-3 2-hydroxy-4-phenylbutanenitrile 
• 99902-37-1 (3R,5R)-5-carbamoyl-3-methyl-7-phenyl-4-oxaheptansaeure 
• 151-50-8 potassium cyanide 
• 75-86-5 2-hydroxy-2-methylpropanenitrile 
• 74-90-8 hydrogen cyanide 
• 2043-61-0 cyclohexanecarbaldehyde 
• 119798-23-1 acetic acid 1-cyano-3-phenylpropyl ester 
• 335087-69-9 (S)-(-)-4-phenyl-2-(trimethylsilyloxy)butanenitrile 
• 18328-11-5 3-benzyl propionaldehyde 

Downstream Products

• 1050264-88-4 (S)-1-cyano-3-phenylpropyl diisopropylcarbamate 
• 887363-68-0 C₁₉H₁₉NO₃ 
• 887363-42-0 C₁₉H₁₉NO₃ 
• 126641-88-1 (R)-(+)-acetoxy-4-phenyl-butanenitrile 
• 90315-82-5 ethyl (R)-2-hydroxy-4-phenylbutyrate 
• 29678-81-7 (R)-2-hydroxy4-phenylbutanoic acid 
• 138566-24-2 (R)-4-Phenyl-2-(tetrahydro-pyran-2-yloxy)-butyronitrile 
• 68844-69-9 (2R)-2-Hydroxy-4-phenylbutyric acid methyl ester 
• 1364488-12-9 (R)-2-cyano-1-ethoxy-1-oxo-4-phenylbutan-2-yl pyrrolidine-1-carboxylate 
• 1364488-16-3 (R)-3-cyano-5,5-dimethyl-4-oxo-1-phenylhexan-3-yl dimethylcarbamate 
• 1364488-15-2 (R)-3-cyano-5-methyl-4-oxo-1-phenylhexan-3-yl dimethylcarbamate 

Relevant articles and documents

A High-Throughput Screening Method for the Directed Evolution of Hydroxynitrile Lyase towards Cyanohydrin Synthesis

Chiral cyanohydrins are useful intermediates in the pharmaceutical and agricultural industries. In nature, hydroxynitrile lyases (HNLs) are a kind of elegant tool for enantioselective hydrocyanation of carbonyl compounds. However, currently available methods for demonstrating hydrocyanation are still stalled at precise, but low-throughput, GC or HPLC analyses. Herein, we report a chromogenic high-throughput screening (HTS) method that is feasible for the cyanohydrin synthesis reaction. This method was highly anti-interference and sensitive, and could be used to directly profile the substrate scope of HNLs either in cell-free extract or fermentation clear broth. This HTS method was also validated by generating new variants of PcHNL5 that presented higher catalytic efficiency and stronger acidic tolerance in variant libraries.

Copper-Catalyzed Substitution of α-Triflyloxy Nitriles and Esters with Silicon Nucleophiles under Inversion of the Configuration

A copper-catalyzed nucleophilic displacement of α-triflyloxy nitriles and esters with silicon nucleophiles allows for the stereospecific generation of highly enantioenriched α-silylated carboxyl compounds. The enantioselective synthesis of α-silylated nitriles is unprecedented. The catalytic system exhibits good functional group tolerance. The stereochemical course of the substitution is shown to proceed with inversion of the configuration. The new reaction is an addition to the still limited number of methods for catalytic C(sp3)-Si cross-coupling.

Improving the properties of bacterial r-selective hydroxynitrile lyases for industrial applications

Hydroxynitrile lyases (HNLs) catalyse the reversible cleavage of cyanohydrins to carbonyl compounds and HCN. The recent discovery of bacterial HNLs with a cupin fold gave rise to a new promising class of these enzymes. They are interesting candidates for the synthesis of cyanohydrins on an industrial scale owing to their high expression levels in Escherichia coli. The activity and enantioselectivity of the manganese-dependent HNL from Granulicella tundricola (GtHNL) were significantly improved by site-saturation mutagenesis of active site amino acids. The combination of beneficial mutations resulted in a variant with 490-fold higher specific activity in comparison to the wild-type enzyme. More importantly, GtHNL-A40H/V42T/Q110H is a highly competitive alternative for the synthesis of chiral cyanohydrins, such as 2-chlorobenzaldehyde cyanohydrin, (R)-2-hydroxy-4-phenylbutyronitrile, and (R)-2-hydroxy-4-phenyl-3-butene nitrile, which serve as intermediates for the synthesis of pharmaceuticals.