124412-00-6 Usage
General Description
5-CFDA, AM (5-Carboxyfluorescein diacetate, acetoxymethyl ester) is a cell-permeant, non-fluorescent compound that is commonly used as a fluorescent probe to measure cell viability and esterase activity. When 5-CFDA, AM enters the cells, it is hydrolyzed by intracellular esterases to yield 5-CFDA, which is membrane-impermeant and green-fluorescent. This allows for the assessment of cell viability and esterase activity in live cells. 5-CFDA, AM is widely used in flow cytometry, fluorescence microscopy, and other cell-based assays to study cell viability, cell proliferation, and cytotoxicity. It is a valuable tool in biomedical research and drug development for assessing the effects of various compounds on cell health and function.
Check Digit Verification of cas no
The CAS Registry Mumber 124412-00-6 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,2,4,4,1 and 2 respectively; the second part has 2 digits, 0 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 124412-00:
(8*1)+(7*2)+(6*4)+(5*4)+(4*1)+(3*2)+(2*0)+(1*0)=76
76 % 10 = 6
So 124412-00-6 is a valid CAS Registry Number.
InChI:InChI=1/C28H20O11/c1-14(29)34-13-35-26(32)17-4-7-21-20(10-17)27(33)39-28(21)22-8-5-18(36-15(2)30)11-24(22)38-25-12-19(37-16(3)31)6-9-23(25)28/h4-12H,13H2,1-3H3