129541-41-9

Basic information

• Product Name: 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt
• Synonyms: 5-BROMO-4-CHLORO-3-INDOLYL B-D-GLUCURONI DE SODIUM;5-Bromo-4-chloro-3-indolylb-D-glucuronidesodiumsalttrihydrate;5-Bromo-4-chloro-3-indolyl-D-glucuronic acid - s;5-BROMO-4-CHLORO-3-INDOLYL-BETA-D -GLUCURONIDE MOLECULAR BIOLOGY REAGENT SODIUM SALT 98+%;5-BROMO-4-CHLORO-3-INDOLYL-BETA-D -GLUCURONIDE SODIUM SALT 89+%;5-BROMO-4-CHLORO-3-INDOLYL-B-D-GLUCURONIDE NA SALT;5-Bromo-4-chloro-3-indolyl-beta-D-glucuronic acid cyclohexylammonium salt >98%;X-GlcA CHA salt, X-Gluc CHA salt
• CAS NO: 129541-41-9
• Molecular Formula: C14H12BrClNNaO7
• Molecular Weight: 444.59
• EINECS: 1592732-453-0
• Product Categories: Carbohydrates & Derivatives;Fluorescent Labels & Indicators;Glucuronides;Labeling and Diagnostics Reagents;substrate
• Mol File: 129541-41-9.mol
• Article Data: 0

Chemical Properties

• Melting Point: 228-230°C
• Boiling Point: 732.2 °C at 760 mmHg
• Flash Point: 396.6 °C
• Appearance: /tablet
• Storage Temp.: −20°C
• Solubility: deionized water: solubleone tablet, 5mg/mL
• Water Solubility: Soluble in water at 10mg/ml
• Sensitive: Light Sensitive
• CAS DataBase Reference: 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt(CAS DataBase Reference)
• NIST Chemistry Reference: 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt(129541-41-9)
• EPA Substance Registry System: 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt(129541-41-9)

Safety Data

• Hazard Codes: Xi
• Statements: 36/37/38
• Safety Statements: 24/25-37/39-26
• WGK Germany: 3
• Hazardous Substances Data: 129541-41-9(Hazardous Substances Data)

Usage

Description

5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt, also known as X-Gluc, is a synthetic compound that serves as a chromogenic substrate for the detection of β-glucuronidase (GUS) enzyme activity. It is a white solid that, upon enzymatic conversion by β-glucuronidase, forms an intense blue precipitate. This property makes it a valuable tool in various applications, particularly in molecular biology and microbiology.

Uses

Used in Molecular Biology:
5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt is used as a detection agent for the Gus gene in molecular biology applications. It is particularly useful for identifying bacterial colonies, such as Escherichia Coli, that express the β-glucuronidase enzyme. The formation of the blue precipitate upon enzymatic conversion allows for easy visualization and selection of colonies with the desired gene expression.
Used in Microbiology:
In microbiology, 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt is used as a substrate for β-glucuronidase, which aids in the identification and differentiation of bacterial strains based on their enzymatic activity. The blue precipitate formed serves as a clear indicator of the presence of the Gus gene in the bacterial colonies.
Used in Histochemical Applications:
5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt is also utilized in histochemical applications for the detection of β-glucuronidase enzyme activity in tissue samples. The intense blue precipitate formed upon enzymatic conversion allows for the visualization of enzyme activity in cells and tissues, providing valuable information for research and diagnostic purposes.
Used in Pharmaceutical Research:
The blue precipitate formed by the enzymatic conversion of 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt can be employed in the development of novel drug delivery systems and pharmaceutical formulations. The clear and easily detectable color change can be used to monitor the release and activity of drugs in various applications, potentially improving the efficacy and bioavailability of therapeutic agents.
Used in Environmental Science:
In environmental science, 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt can be used to study the presence and activity of β-glucuronidase-producing microorganisms in various ecosystems. The blue precipitate formed can serve as an indicator of the presence of these microorganisms, providing insights into their role in environmental processes and potential applications in bioremediation and other areas.

Biochem/physiol Actions

5-bromo-4-chloro-3-indolyl-β-D-glucuronide sodium salt (X-GlcA), a colorless substrate for β-glucuronidase enzyme, generates a blue color at the end of the reaction.

InChI:InChI=1/C14H13BrClNO7.Na/c15-4-1-2-5-7(8(4)16)6(3-17-5)23-14-11(20)9(18)10(19)12(24-14)13(21)22;/h1-3,9-12,14,17-20H,(H,21,22);/q;+1/p-1/t9-,10+,11+,12-,14+;/m1./s1