13111-32-5Relevant articles and documents
NADP-dependent glutamate dehydrogenases in a dimorphic zygomycete Benjaminiella poitrasii: Purification, characterization and their evaluation as an antifungal drug target
Deshpande, Mukund V.,Kulkarni, Anand M.,Pathan, Ejaj K.,Prasanna, Nallaballe V. L.,Ramana, Chepuri V.
, (2020)
Background: It has been reported that the genes coding for NADP-dependent glutamate dehydrogenases (NADP-GDHs) showed a cause-effect relationship with Yeast-Hypha (Y[sbnd]H) reversible transition in a zygomycete Benjaminiella poitrasii. As Y[sbnd]H transition is significant in human pathogenic fungi for their survival and proliferation in the host, the NADP-GDHs can be explored as antifungal drug targets. Methods: The yeast-form specific BpNADPGDH I and hyphal-form specific BpNADPGDH II of B. poitrasii were purified by heterologous expression in E. coli BL-21 cells and characterized. The structural analogs of L-glutamate, dimethyl esters of isophthalic acid (DMIP) and its derivatives were designed, synthesized and screened for inhibition of NADP-GDH activity as well as Y[sbnd]H transition in B. poitrasii, and also in human pathogenic Candida albicans strains. Results: The BpNADPGDH I and BpNADPGDH II were found to be homo-hexameric proteins with native molecular mass of 282 kDa and 298 kDa, respectively and subunit molecular weights of 47 kDa and 49 kDa, respectively. Besides the distinct kinetic properties, BpNADPGDH I and BpNADPGDH II were found to be regulated by cAMP-dependent- and Calmodulin (CaM) dependent- protein kinases, respectively. The DMIP compounds showed a more pronounced effect on H-form specific BpNADPGDH II and inhibited Y[sbnd]H transition as well as growth in B. poitrasii and C. albicans strains. Conclusion: The present study will be useful to design and develop antifungal drugs against dimorphic human pathogens using glutamate dehydrogenase as a target. Significance: Glutamate dehydrogenases can be explored as a target against human pathogenic fungi.
Enhanced Carboxylate Binding Using Urea Amide-Based Receptors with Internal Lewis Acid Coordination: A Cooperative Polarization Effect
Hughes, Martin Patrick,Smith, Bradley D.
, p. 4492 - 4499 (1997)
A structural design strategy is described that greatly improves the acetate binding ability of neutral urea and amide-based receptors. The enhanced binding is due to a cooperative polarization effect which is induced by intramolecular coordination of the
General method for quickly synthesizing bridge-linked bisamide, triamide and tetraamide derivatives through ultrasonic radiation method and application
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Paragraph 0057-0059, (2017/07/14)
The invention belongs to the technical field of organic synthesis, specifically discloses bridge-linked bisamide, triamide and tetraamide derivatives and a general method for ultrasonic radiation quick synthesis thereof, and also relates to the applicatio
Aromatic isophthalamides aggregate in lipid bilayers: Evidence for a cooperative transport mechanism
Berry, Stuart N.,Busschaert, Nathalie,Frankling, Charlotte L.,Salter, Dale,Gale, Philip A.
supporting information, p. 3136 - 3143 (2015/04/27)
The synthesis and anion transport properties of a series of transmembrane anion transporters based on an isophthalamide scaffold with phenyl, naphthyl or anthracenyl central rings are reported. Anion transport studies using POPC vesicles, showed that the
