1492-30-4 Usage
Description
4-Nitrophenyl palmitate, also known as p-Nitrophenyl palmitate, is a synthetic ester compound that serves as a substrate for lipase enzyme activity. It is formed by the condensation of the carboxy group of palmitic acid with the phenolic hydroxy group of p-nitrophenol. 4-NITROPHENYL PALMITATE is widely used in the study and analysis of lipase enzyme activity due to its unique properties and the ease of measuring the reaction product.
Uses
Used in Enzyme Activity Analysis:
4-Nitrophenyl palmitate is used as a substrate for lipase enzyme activity assays. The lipase enzyme hydrolyzes 4-nitrophenyl palmitate, yielding the yellow-colored product 4-nitrophenol, which can be measured spectrophotometrically at 410 nm. This application is valuable for researchers and scientists studying lipase activity, enzyme kinetics, and enzyme inhibition, as it allows for quick and accurate measurements of enzyme activity.
Used in Immobilization Methods:
In the field of biochemistry and biotechnology, 4-nitrophenyl palmitate is used as a substrate in the immobilization method of lipase on the interface of natural polysaccharide particles. This method is advantageous due to its short reaction time and the ease of performing spectrophotometric analyses, making it a preferred choice for studying enzyme immobilization and its effects on enzyme activity.
Used in Pharmaceutical Research:
4-Nitrophenyl palmitate is also utilized in pharmaceutical research as a tool to study the interactions between lipase enzymes and potential drug candidates. By understanding how these enzymes interact with various compounds, researchers can develop more effective drugs for treating lipase-related conditions and diseases.
Used in Analytical Chemistry:
In analytical chemistry, 4-Nitrophenyl palmitate is employed as a reference compound for the development and validation of new methods and techniques for enzyme activity analysis. Its well-defined properties and the ease of measuring its reaction product make it an ideal candidate for this purpose, allowing for the accurate assessment of new analytical methods and their effectiveness in measuring lipase activity.
Check Digit Verification of cas no
The CAS Registry Mumber 1492-30-4 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 1,4,9 and 2 respectively; the second part has 2 digits, 3 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 1492-30:
(6*1)+(5*4)+(4*9)+(3*2)+(2*3)+(1*0)=74
74 % 10 = 4
So 1492-30-4 is a valid CAS Registry Number.
InChI:InChI=1/C22H35NO4/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-22(24)27-21-18-16-20(17-19-21)23(25)26/h16-19H,2-15H2,1H3
1492-30-4Relevant articles and documents
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Murakami,Y. et al.
, p. 24 - 32 (1977)
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Enzyme mediated-transesterification of verbascoside and evaluation of antifungal activity of synthesised compounds
Khazir, Jabeena,Ali, Intizar,Khan, Inshad Ali,Sampath Kumar, Halmuthur Mahabalarao
, p. 727 - 734 (2015/09/23)
Enzymatic acylation of verbascoside, a polyhydroxylated natural product, has been reported in this study using five different commercial lipases and taking p-nitrophenyl alkanoates as acyl donors. Out of these enzymes, the immobilised Candida antarctica lipase B was found as the enzyme of choice. Mono-and di-acylated products were formed, with mono as major product indicating high regioselective nature of such transformations. A series of acyl esters of verbascoside have been synthesised by this enzymatic transesterification methodology. The lipophilicity of the synthesised analogues was also checked. The analogues were further subjected to synergistic antifungal activity with amphotericin B (AmB) against Candida albicans. Fourfold reduction in minimum inhibitory concentration of AmB was observed with few synthesised analogues such as verbascoside 4″-octanoate (3b), verbascoside 4″-palmitate (3d) and verbascoside 4″,4′-dipalmitate (4d) at a concentration of 0.5 g/mL.
Synthesis of Ceramide Analogues Having the C(4)-C(5) Bond of the Long-Chain Base as Part of an Aromatic or Heteroaromatic System
Chun, Jiong,He, Linli,Byun, Hoe-Sup,Bittman, Robert
, p. 7634 - 7640 (2007/10/03)
Two efficient and stereoselective methods are described for the preparation of aryl and heteroaryl ceramide analogues 2 and 3. The first route involves the addition of an aryllithium or a heteroaryllithium reagent (7a or 25a, respectively) to the L-serine-derived aldehyde 4, followed by hydrolysis of the oxazolidine, liberation of the amino group, and N-acylation. The second route, which was used to prepare arylceramide analogue 2 in eight steps and 28% overall yield starting with 3-bromobenzaldehyde, utilizes a Heck reaction to afford (E)-α,β-unsaturated ester 16, then osmium-catalyzed asymmetric dihydroxylation for the introduction of the desired chirality at C-2 and C-3. Regioselective α-azidation of α-O-nosyl-β-hydroxyester 18 with sodium azide, followed by LiAlH4 reduction of the azido and ester groups and N-acylation, complete the synthesis of arylceramide analogue 2.
