14948-96-0 Usage
Description
4-Nitrophenyl-N-acetyl-beta-D-galactosaminide, also known as p-Nitrophenyl 2-Acetamido-2-deoxy-β-D-galactopyranoside, is a chromogenic substrate used in the study and analysis of specific enzymes. It is a crystalline compound with a 4-nitrophenyl substituent at the anomeric position, which allows for the detection and quantification of enzymatic activity.
Uses
Used in Enzyme Assays:
4-Nitrophenyl-N-acetyl-beta-D-galactosaminide is used as a substrate for enzyme assays, specifically for N-acetyl-beta-D-galactosaminidase and N-Acetyl-beta-D-hexosaminidase. The compound serves as a tool to study the activity, kinetics, and properties of these enzymes, which are involved in the metabolism of complex carbohydrates and the degradation of glycoconjugates.
Used in Research and Development:
In the field of research and development, 4-Nitrophenyl-N-acetyl-beta-D-galactosaminide is used as a compound in organic synthesis. Its unique structure and properties make it a valuable component in the development of new chemical entities and the study of various biological processes.
Used in Pharmaceutical Industry:
4-Nitrophenyl-N-acetyl-beta-D-galactosaminide is used as a reagent in the pharmaceutical industry for the development and testing of new drugs targeting enzymes involved in carbohydrate metabolism and related diseases. Its ability to serve as a substrate for specific enzymes makes it a useful tool in the design and optimization of potential therapeutic agents.
Used in Diagnostics:
In the diagnostics industry, 4-Nitrophenyl-N-acetyl-beta-D-galactosaminide is used as a component in the development of diagnostic tests for various conditions related to enzyme deficiencies or abnormalities in carbohydrate metabolism. The compound's ability to act as a substrate for specific enzymes allows for the detection and quantification of enzymatic activity, which can be indicative of certain diseases or disorders.
Check Digit Verification of cas no
The CAS Registry Mumber 14948-96-0 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,4,9,4 and 8 respectively; the second part has 2 digits, 9 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 14948-96:
(7*1)+(6*4)+(5*9)+(4*4)+(3*8)+(2*9)+(1*6)=140
140 % 10 = 0
So 14948-96-0 is a valid CAS Registry Number.
14948-96-0Relevant articles and documents
Scope of the DMC mediated glycosylation of unprotected sugars with phenols in aqueous solution
Fairbanks, Antony J.,Qiu, Xin
, p. 7355 - 7365 (2020/10/13)
Activation of reducing sugars in aqueous solution using 2-chloro-1,3-dimethylimidazolinium chloride (DMC) and triethylamine in the presence of para-nitrophenol allows direct stereoselective conversion to the corresponding 1,2-Trans para-nitrophenyl glycosides without the need for any protecting groups. The reaction is applicable to sulfated and phosphorylated sugars, but not to ketoses or uronic acids or their derivatives. When applied to other phenols the product yield was found to depend on the pKa of the added phenol, and the process was less widely applicable to 2-Acetamido sugars. For 2-Acetamido substrates an alternative procedure in which the glycosyl oxazoline was pre-formed, the reaction mixture freeze-dried, and the crude product then reacted with an added phenol in a polar aprotic solvent system with microwave irradiation proved to be a useful simplification.
Syntheses of the 3- and 4-thio analogues of 4-nitrophenyl 2-acetamido-2-deoxy-β-d-gluco- and galactopyranoside
Chen, Hong-Ming,Withers, Stephen G.
, p. 2212 - 2222 (2008/02/12)
The syntheses of 4-nitrophenyl β-glycosides of the 3-thio and 4-thio analogues of the two principal 2-acetamido-2-deoxy-hexoses found in living systems, GlcNAc and GalNAc, are described. While synthesis of the 4-thio analogues could be achieved via nucleophilic displacements of sulfonate derivatives with thioacetate, problems with neighbouring group acetamido participation necessitated the use of sulfamidate intermediates for the 3-thio analogues. These 3- and 4-thio analogues are employed in the chemo-enzymatic synthesis of thio-oligosaccharide analogues of structures present in glycosaminoglycans, glycoproteins and glycolipids.
