1733-12-6 Usage
Description
Cresol Red is a member of the class of 2,1-benzoxathioles, specifically 2,1-benzoxathiole 1,1-dioxide, in which both of the hydrogens at position 3 have been substituted by 4-hydroxy-5-methylphenyl groups. It is a dark green or brown-red powder and is known for its use as an acid-base indicator.
Uses
Used in Acid-Base Indicator Applications:
Cresol Red is used as an acid-base indicator, with transition intervals of pH 1.8 (orange) to pH 2.0 (yellow) and pH 7.0 (yellow) to pH 8.8 (violet). This property makes it useful for detecting changes in pH levels in various chemical and biological processes.
Used in Detection of Urease:
Cresol Red is commonly used with lead acetate for the detection of urease after polyacrylamide gel electrophoresis (PAGE). This application is valuable in the study of enzymatic activity and related research.
Used in Electrophoresis as a Tracking Dye:
Cresol Red is utilized as a tracking dye in DNA, RNA (agarose), and protein (polyacrylamide) electrophoresis. In agarose, Cresol Red runs with an apparent molecular size of approximately 125 base pair (bp) DNA. Importantly, Cresol Red does not inhibit Taq polymerase, making it a suitable choice for electrophoresis applications without interfering with the polymerase activity.
Purification Methods
Crystallise it from glacial acetic acid. Dry it in air. Dissolve it in aqueous 5% NaHCO3 solution and precipitate it from a hot solution by dropwise addition of aqueous HCl. Repeat the procedure till the UVmax does not increase. [Beilstein 19 IV 1133.]
Check Digit Verification of cas no
The CAS Registry Mumber 1733-12-6 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 1,7,3 and 3 respectively; the second part has 2 digits, 1 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 1733-12:
(6*1)+(5*7)+(4*3)+(3*3)+(2*1)+(1*2)=66
66 % 10 = 6
So 1733-12-6 is a valid CAS Registry Number.
InChI:InChI=1/C21H18O5S/c1-13-11-15(7-9-18(13)22)21(16-8-10-19(23)14(2)12-16)17-5-3-4-6-20(17)27(24,25)26/h3-12,22H,1-2H3,(H,24,25,26)/b21-16-
1733-12-6Relevant articles and documents
Solvent-free one-pot synthesis of sulfonephthaleins from saccharin and phenols
Tillu,Dumbre,Borate,Wakharkar,Choudhary
experimental part, p. 1101 - 1107 (2012/05/05)
Sulfonephthaleins can be synthesized in a single pot from saccharin and phenol via the in situ formation of 2-sulfobenzoic anhydride, followed by its reaction with phenol using H2SO4 as the condensing agent, in the absence of any solvent. This solvent-free synthesis is more economical and environmentally benign. Copyright Taylor & Francis Group, LLC.