181259-63-2Relevant articles and documents
Preparation of (2?-5?)-oligonucleotides based on 6-N- benzylaminopurineriboside using the Spicaria violacea fungal enzyme complex
Kulak,Tkachenko,Grishan,Kukharskaya,Eroshevskaya,Kalinichenko,Zinchenko
experimental part, p. 74 - 78 (2009/07/18)
A method for chemico-enzymatic synthesis of (2?-5?)-oligonucleotides with 6-N-benzylaminopurineriboside as the nucleoside units was proposed. The method consisted of enzymatic hydrolysis of the oligonucleotides with mixed (2?-5?)-(3?-5?)-phosphodiesterbonds that were prepared by polymerization of 6-N-benzyladenosine-2?(3?)-monophosphate by using (3?-5?)-specific nuclease and phosphatase contained in the filtrate of culture medium of the mycelial fungus Spicaria violacea.
N6-benzyladenosine analogs of (A2′p)2A: Synthesis and activity to tobacco mosaic virus
Kvasyuk,Kulak,Zinchenko,Barai,Mikhailopulo
, p. 181 - 187 (2007/10/03)
Analogs of (2′-5′)oligoadenylate trimer with N6-benzyladenosine in various positions of the chain and the fully substituted trimer were synthesized by the phosphotriester method. The structures of compounds prepared were proved by UV, CD, and 1H NMR. The obtained substances inhibit replication of tobacco mosaic virus at a 10-8-10-6 M concentration, which is comparable with that of natural (A2′p)2A triadenylate.