18656-96-7

Basic information

• Product Name: 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt
• Synonyms: 5-BROMO-4-CHLORO-3-INDOXYL-BETA-D-GLUCOPYRANOSIDE;GLUC(X-);X-GLUC;X-GLU;X-BETA-D-GLC;X-BETA-D-GLUCOSIDE;5-BROMO-3-INDOLYL-B-D-GLUCURONIDECYCLOHE XYLAMMONIU;NALGENE CENTRIFUGE BOTTLE STYLE 3122, 29 0 ML
• CAS NO: 18656-96-7
• Molecular Formula: C20H26BrClN2O7
• Molecular Weight: 521.79
• EINECS: 239-603-0
• Product Categories: Enzyme substrates
• Mol File: 18656-96-7.mol
• Article Data: 0

Chemical Properties

• Melting Point: 249 °C
• Boiling Point: 742 °C at 760 mmHg
• Flash Point: 402.6 °C
• Appearance: /
• Vapor Pressure: 8.69E-23mmHg at 25°C
• Storage Temp.: −20°C
• Solubility: DMF: 50 mg/mL, clear, colorless to very faintly yellow
• CAS DataBase Reference: 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt(CAS DataBase Reference)
• NIST Chemistry Reference: 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt(18656-96-7)
• EPA Substance Registry System: 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt(18656-96-7)

Safety Data

• WGK Germany: 3
• F: 8-10-21
• Hazardous Substances Data: 18656-96-7(Hazardous Substances Data)

Usage

General Description

5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt is a chemical compound used in various biochemical and molecular biology applications. It is a substrate for beta-glucuronidase, an enzyme commonly used as a marker for gene expression in molecular biology research. The compound is often employed in colorimetric and histochemical assays to detect the presence and activity of beta-glucuronidase, which cleaves the chromogenic substrate to produce a blue coloration. This makes it useful for the visualization and quantification of gene expression and enzyme activity. The cyclohexylammonium salt form of 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide is soluble in organic solvents and can be used in a variety of experimental protocols for the study of gene regulation and expression.

InChI:InChI=1/C18H21BrClNO7.C6H13N/c1-15(24)13(14(22)23)28-18(4,17(3,26)16(15,2)25)27-10-7-21-9-6-5-8(19)12(20)11(9)10;7-6-4-2-1-3-5-6/h5-7,13,21,24-26H,1-4H3,(H,22,23);6H,1-5,7H2/t13?,15-,16u,17u,18-;/m1./s1