219709-48-5

Basic information

• Product Name: 4-S-pivaloyloxymethyl-2'-O-tert-butyldimethylsilyl-5'-O-dimethoxytrityl-4-thiouridine
• CAS NO: 219709-48-5
• Molecular Weight: 791.05
• Mol File: 219709-48-5.mol
• Article Data: 1

Chemical Properties

• CAS DataBase Reference: 4-S-pivaloyloxymethyl-2'-O-tert-butyldimethylsilyl-5'-O-dimethoxytrityl-4-thiouridine(CAS DataBase Reference)
• NIST Chemistry Reference: 4-S-pivaloyloxymethyl-2'-O-tert-butyldimethylsilyl-5'-O-dimethoxytrityl-4-thiouridine(219709-48-5)
• EPA Substance Registry System: 4-S-pivaloyloxymethyl-2'-O-tert-butyldimethylsilyl-5'-O-dimethoxytrityl-4-thiouridine(219709-48-5)

Safety Data

• Hazardous Substances Data: 219709-48-5(Hazardous Substances Data)

Upstream product

• 136671-59-5 5'-O-dimethoxytrityl-4-thiouridine 
• 219709-47-4 4-S-pivaloyloxymethyl-5'-O-dimethoxytrityl-3'-O-tert-butyldimethylsilyl-4-thiouridine 
• 18997-19-8 Chloromethyl pivalate 
• 18162-48-6 tert-butyldimethylsilyl chloride 
• 13957-31-8 4-thiouridine 
• 40615-36-9 4,4'-dimethoxytrityl chloride 

Relevant articles and documents

Chemical synthesis of 4-thiouridine-containing substrate analogues of tRNA:pseudouridine-55 synthase: Photocross-linking studies

For site specific incorporation of 4-thiouridine into oligoribonucleotides a new phosphoramidite is proposed. It makes use of the S-pivaloyloxymethyl group for the protection of the thiol function. This group is easily introduced and removed without modification of the standard protocol for solid phase synthesis of RNA. Three 4-thiouridine-containing oligoribonucleotides (21-mers), corresponding to tRNA minisubstrates of yeast tRNA:pseudouridine-55 synthase (Pus4) were prepared. These 4-thiouridine containing substrates were characterized and used as photoaffinity probe of the enzyme:substrate complex. Irradiation resulted in the specific photocross-linking of these oligoribonucleotides with purified recombinant tRNA:pseudouridine-55 synthase.