23109-05-9

Basic information

• Product Name: ALPHA-AMANITIN
• Synonyms: Aids004473;Aids-004473;AMANITIN, a-(RG);AMANITIN, a-(RG)(CALL);cyclo[L-Asparaginyl-4-hydroxy-L-proly-(R-4,5-dihydroxy-L-isoleucyl-6-hydroxy-2-mercapto-L-tryptophylglycyl-L-isoleucylglycyl-L-cysteinyl]cyclic(4-8)-sulfide(R)-S-oxide;alpha-AMantine, froM AManita Phalloides;AMANITIN, A-;ALPHA-AMANITIN
• CAS NO: 23109-05-9
• Molecular Formula: C39H54N10O14S
• Molecular Weight: 918.97
• EINECS: 245-432-2
• Product Categories: inhibitor;Alkaloids;DNA-RNA Transcription RegulatorsCell Signaling and Neuroscience;Gene Regulation and Expression;Cell Signaling and Neuroscience;Mushroom;Toxins and Venoms;Enzyme Inhibitors;Enzyme Inhibitors by Type;Other;Miscellaneous Enzyme
• Mol File: 23109-05-9.mol
• Article Data: 0

Chemical Properties

• Melting Point: 254-255 °C(lit.)
• Boiling Point: 1622.182 °C at 760 mmHg
• Flash Point: 934.862 °C
• Appearance: white to light yellow/powder
• Density: 1.1626 (rough estimate)
• Refractive Index: 1.7400 (estimate)
• Storage Temp.: −20°C
• Solubility: H2O: 1.0 mg/mL
• PKA: 9.63±0.70(Predicted)
• Merck: 13,370
• BRN: 1071138
• CAS DataBase Reference: ALPHA-AMANITIN(CAS DataBase Reference)
• NIST Chemistry Reference: ALPHA-AMANITIN(23109-05-9)
• EPA Substance Registry System: ALPHA-AMANITIN(23109-05-9)

Safety Data

• Hazard Codes: T+
• Statements: 26/27/28-33-26/28
• Safety Statements: 36/37/39-45-36/37-28
• RIDADR: UN 3462 6.1/PG 2
• WGK Germany: 3
• RTECS: BD6195000
• F: 8-10-18-21
• HazardClass: 6.1(a)
• PackingGroup: I
• Hazardous Substances Data: 23109-05-9(Hazardous Substances Data)

Usage

Description

ALPHA-AMANITIN is a naturally occurring cyclic octapeptide toxin derived from the Amanita phalloides mushroom, also known as the death cap. It is a potent inhibitor of RNA polymerase II, which plays a crucial role in the transcription process of eukaryotic cells. Due to its high toxicity and specific mode of action, ALPHA-AMANITIN has found applications in various research and experimental settings.

Uses

Used in Cellular and Molecular Biology Research:
ALPHA-AMANITIN is used as a tool for inducing transcriptional arrest in NT2 cells prior to immunofluorescence assay. This application allows researchers to study the effects of transcriptional inhibition on cellular processes and gene expression.
Used in Nephrotoxicity Studies:
In the field of toxicology, ALPHA-AMANITIN is used to induce nephrotoxicity in mice renal tissues. This helps researchers understand the mechanisms of kidney damage and develop potential therapeutic strategies to counteract or mitigate the toxic effects of various substances on the kidneys.
Used in Genotoxicity Assessment:
ALPHA-AMANITIN is employed in genotoxicity studies to induce and analyze genotoxic effects in mice bone marrow cells. This is achieved through various assays, such as cell viability assay, comet assay, and chromosomal aberration assay. These studies contribute to the understanding of the potential carcinogenic and mutagenic effects of ALPHA-AMANITIN and other substances on the genetic material of cells.

Biological Activity

α-amanitin, the most deleterious toxin of a. phalloides to humans, inhibits rna polymerase ii (rnapii), causing hepatic and renal failure.

Biochem/physiol Actions

The major toxic constituent of the mushroom, Amanita phalloides, inhibits eukaryotic RNA polymerase II and III, but does not inhibit RNA polymerase I or bacterial RNA polymerase. Inhibits mammalian protein synthesis.