2465-27-2 Usage
Description
AURAMINE O is a hydrochloride obtained by combining 4,4'-carbonimidoylbis(N,N-dimethylaniline) with one molar equivalent of hydrogen chloride. It is a fluorescent stain used for demonstrating acid-fast organisms in a method similar to the Ziehl-Neelsen. It also can be used to make a fluorescent Schiff reagent. AURAMINE O is a yellow crystalline powder or flaky material with a bright yellowish shade.
Uses
Used in Fluorescence Microscopy:
AURAMINE O is used as a fluorescent stain for acid-fast bacilli on paraffin sections in Churukian's modification of Truant's fluorescent method.
Used in Medical Diagnostics:
AURAMINE O is used as a fluorescent stain for acid-resistant bacteria in sputum samples, aiding in the detection and identification of specific bacterial infections.
Used in Textile Industry:
AURAMINE O is used as a dye in the textile industry for its bright yellowish color, with a tinting strength of 100-105%.
Used in Chemical Analysis:
AURAMINE O is used in chemical analysis for its heat resistance of at least 280°C, density of 1.07 g/cm3, and water solubility of at least 10 g/L at 20°C.
Air & Water Reactions
Insoluble in water.
Reactivity Profile
AURAMINE O is incompatible with strong oxidizing agents.
Hazard
Probable carcinogen.
Fire Hazard
Flash point data for AURAMINE O are not available. AURAMINE O is probably combustible.
Potential Exposure
Auramine is used industrially as a dye
or dye intermediate for coloring textiles; paper, and leather.
Also used as an antiseptic (a powerful antiseptic in ear and
nose surgery, and treatment of gonorrhea) and fungicide.
Human exposure to auramine occurs principally through
skin absorption or inhalation of vapors. Low-level dermal
exposure to the consumer may occur but would be limited
to any migration of auramine from fabric, leather, or paper
goods.
Shipping
UN2811 Toxic solids, organic, n.o.s., Hazard
Class: 6.1; Labels: 6.1-Poisonous materials, Technical
Name Required. UN3143 Dyes, solid, toxic, n.o.s. or Dye
intermediates, solid, toxic, n.o.s., Hazard Class: 6.1;
Labels: 6.1-Poisonous materials, Technical Name Required.
TEST ITEMS
SPECIFICATION
HEAT RESISTANCE
280 °C min
DENSITY
1.07 g/cm
3
WATER FASTNESS
4-5
LIGHT FASTNESS
1-2
BLEACHABILITY (OXIDATIVE)
4
BLEACHABILITY (REDUCTIVE)
3
WATER SOLUBILITY AT
20
°C
10 g/L min
WATER INSOLUBLE
1.0% max
MOISTURE
3.0% max
WEIGHT METAL TOTAL
50ppm max
Purification Methods
It crystallises from EtOH as the hydrochloride and is very slightly soluble in CHCl3, UV: 434nm ( 370). The free base (carbinolamine) has m 136o (from *C6H6). [Goldacre & Phillips J Chem Soc 1724 1949, Conrad et al. Biochemistry 9 1540 1970, Beilstein 14 IV 256.]
Incompatibilities
Incompatible with oxidizers (chlorates,
nitrates, peroxides, permanganates, perchlorates, chlorine,
bromine, fluorine, etc.); contact may cause fires or explosions.
Keep away from alkaline materials, strong bases,
strong acids, oxoacids, epoxides.
Waste Disposal
Consult with environmental
regulatory agencies for guidance on acceptable disposal
practices. Generators of waste containing this contaminant
(≥100 kg/mo) must conform with EPA regulations governing
storage, transportation, treatment, and waste disposal.
Incinerate in furnace with afterburner and scrubber.
Check Digit Verification of cas no
The CAS Registry Mumber 2465-27-2 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 2,4,6 and 5 respectively; the second part has 2 digits, 2 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 2465-27:
(6*2)+(5*4)+(4*6)+(3*5)+(2*2)+(1*7)=82
82 % 10 = 2
So 2465-27-2 is a valid CAS Registry Number.
InChI:InChI=1/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H
