252039-52-4Relevant articles and documents
Effect of laser and LED on enzymatic production of ceramide
Zhang, Hongyu,Zhang, Long,Tidemand-Lichtenberg, Peter,Buchhave, Preben,Xu, Xuebing,Li, Yingxin
, p. 131 - 136 (2011)
An enzyme (Phospholipase C Type I from Clostridium perfringens) was exposed to 0-810 J cm-2 of energy using laser light at wavelengths 808, 532, 1064 and 1342 nm and two LED light sources at wavelengths 810 and 640 nm. Enzyme responses were evaluated by measuring ceramide concentration using high performance thin-layer chromatography (HPTLC) at 0.5, 1, 2, 3, 4, 6, 17, 24 h after irradiation. The duration of effect was evaluated from the experimental data. The results show that enzyme activity can be increased by using both laser and LED sources whose wavelength is located within a certain range. The effect depends on the energy and wavelength of the light. The increase in enzyme activity continued for about 4 h after irradiation. This study shows that the duration of irradiation should be included as one of the main laser parameters when reporting on the effects of laser irradiation on enzymes. We also find that laser sources and LED sources have the same effect on enzyme activity if the wavelength and absorbed energy are equal. 2010 Tianjin Medical University. Photochemistry and Photobiology
Chiral combinatorial preparation and biological evaluation of unique ceramides for inhibition of sphingomyelin synthase
Koolath, Sajeer,Monde, Kenji,Murai, Yuta,Suga, Yoshiko
supporting information, (2020/02/04)
Enantiomers or diastereomers of chiral bioactive compounds often exhibit different biological and toxicological properties. Here, we report the efficient synthesis of four stereoisomers of sphingosine and derivatization of unique chiral ceramides through a combinatorial chemistry by solid-phase activated resin ester. In addition, to test the effectivity of stereochemistry of ceramide, we demonstrated a cell-based assay of sphingomyelin synthase inhibition in the presence ofchiral unique ceramides, which suggested that libraries of this sort will be a rich source of biologically active synthetic molecules.
Design and synthesis of sphingomyelin-cholesterol conjugates and their formation of ordered membranes
Matsumori, Nobuaki,Tanada, Norio,Nozu, Kohei,Okazaki, Hiroki,Oishi, Tohru,Murata, Michio
, p. 8568 - 8575 (2011/09/15)
A lipid raft is a cholesterol (Chol)-rich microdomain floating in a sea of lipid bilayers. Although Chol is thought to interact preferentially with sphingolipids such as sphingomyelin (SM), rather than with glycerophospholipids, the origin of the specific interaction has remained unresolved, primarily because of the high mobility of lipid molecules and weak intermolecular interactions. In this study, we synthesized SM-Chol conjugates with functionally designed linker portions to restrain Chol mobility and examined their formation of ordered membranes by a detergent insolubility assay, fluorescence anisotropy experiments, and fluorescence-quenching assay. In all of the tests, membranes prepared from the conjugates showed properties of ordered domains comparable to a SM-Chol (1:1) membrane. To gain insight into the structure of bilayers composed from the conjugates, we performed molecular dynamics simulations with 64 molecules of the conjugates, which suggested that the conjugates form a stable bilayer structure by bending at the linker portion and, mostly, reproduce the hydrogen bonds between the SM and Chol portions. These results imply that the molecular recognition between SM and Chol in an ordered domain is essentially reproduced by the conjugated molecules and, thus, demonstrates that these conjugate molecules could potentially serve as molecular probes for understanding molecular recognition in lipid rafts.
