25266-13-1Relevant articles and documents
A ε - ester perfume compound and its preparation method
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Paragraph 0031-0033, (2017/11/17)
The invention discloses an epsilon-lactone flavor chemical compound. The epsilon-lactone flavor chemical compound is shown as a structural formula. The invention further discloses a method for preparing the epsilon-lactone flavor chemical compound. The method includes enabling acid chemical compounds and alcohol chemical compounds to carry out esterification reaction in organic solvents by the aid of acid catalysts to obtain acrylic acid n-octyl chemical compounds; enabling the acrylic acid n-octyl chemical compounds and alkenes chemical compounds to carry out addition and Baeyer-Villiger oxidation reaction in organic solvents by the aid of base catalysts and acid catalysts to ultimately obtain the epsilon-propanoic acid n-octyl-epsilon-caprolactone chemical compound. The epsilon-lactone flavor chemical compound and the method have the advantages that the epsilon-lactone flavor chemical compound is a novel epsilon-lactone chemical compound with characteristic aroma of mellow and sweet rice wine, and is the latest domestic and overseas research result for epsilon-lactone chemical compounds with characteristic aroma.
Non-Viral CRISPR/Cas Gene Editing In Vitro and In Vivo Enabled by Synthetic Nanoparticle Co-Delivery of Cas9 mRNA and sgRNA
Miller, Jason B.,Zhang, Shuyuan,Kos, Petra,Xiong, Hu,Zhou, Kejin,Perelman, Sofya S.,Zhu, Hao,Siegwart, Daniel J.
supporting information, p. 1059 - 1063 (2017/01/18)
CRISPR/Cas is a revolutionary gene editing technology with wide-ranging utility. The safe, non-viral delivery of CRISPR/Cas components would greatly improve future therapeutic utility. We report the synthesis and development of zwitterionic amino lipids (ZALs) that are uniquely able to (co)deliver long RNAs including Cas9 mRNA and sgRNAs. ZAL nanoparticle (ZNP) delivery of low sgRNA doses (15 nm) reduces protein expression by >90 % in cells. In contrast to transient therapies (such as RNAi), we show that ZNP delivery of sgRNA enables permanent DNA editing with an indefinitely sustained 95 % decrease in protein expression. ZNP delivery of mRNA results in high protein expression at low doses in vitro (?1). Intravenous co-delivery of Cas9 mRNA and sgLoxP induced expression of floxed tdTomato in the liver, kidneys, and lungs of engineered mice. ZNPs provide a chemical guide for rational design of long RNA carriers, and represent a promising step towards improving the safety and utility of gene editing.
PROCESS FOR PREPARING 4-HYDROXYBUTYL ACRYLATE
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Paragraph 0549; 0550; 0551; 0552; 0553; 0554; 0555; 0556, (2015/04/22)
A process for preparing 4-hydroxybutyl acrylate by transesterifying an alkyl acrylate with 1,4-butanediol in the presence of a dialkyltin oxide such that each of the alkyl groups has 4 to 8 carbon atoms, characterized in that the amount of the dialkyltin oxide is adjusted to 0.00001 to 0.01 moles per one mole of the alkyl acrylate.