2650-17-1

Basic information

• Product Name: Xylene Cyanole FF
• Synonyms: 1,3-benzenedisulfonicacid,4-[[4-(ethylamino)-3-methylphenyl][4-(ethylimino)-3;Xylene Gyanol FF;Acid blue 147 (C.I. 42135);Sodium hydrogen 4-[[4-(ethylamino)-m-tolyl][4-(ethylimino)-3-methylcyclohexa-2,5-dien-1-ylidene]methyl]benzene-1,3-disulphonate;Xylenecyanol FF, dye content 70%;Acid blue 147, Cyanol FF, XC, Xylene CyanoleFF;Acid blue 147, Cyanol FF, XC;1,3-Benzenedisulfonic acid, 4-[[4-(ethylamino)-3-methylphenyl][4-(ethylimino)-3-methyl-2,5-cyclohexadien-1-ylidene]methyl]-, sodium salt (1:1)
• CAS NO: 2650-17-1
• Molecular Formula: C25H27N2NaO6S2
• Molecular Weight: 538.61
• EINECS: 224-728-5
• Mol File: 2650-17-1.mol
• Article Data: 0

Chemical Properties

• Melting Point: 295°C
• Appearance: Green/Solid
• Storage Temp.: Store at RT.
• Solubility: H2O: complete1mg/mL, clear, blue
• Water Solubility: Soluble in water.
• Stability: Stable. Moisture-sensitive. Incompatible with strong oxidizing agents.
• CAS DataBase Reference: Xylene Cyanole FF(CAS DataBase Reference)
• NIST Chemistry Reference: Xylene Cyanole FF(2650-17-1)
• EPA Substance Registry System: Xylene Cyanole FF(2650-17-1)

Safety Data

• Hazard Codes: Xi
• Statements: 36/37/38
• Safety Statements: 26-36-24/25
• WGK Germany: 3
• TSCA: Yes
• Hazardous Substances Data: 2650-17-1(Hazardous Substances Data)

Usage

Description

Xylene Cyanol FF, also known as Xylene Cyanol, is a synthetic organic dye that exhibits a bright blue color when dissolved in water. It is commonly used as a tracking dye in various applications, particularly in the field of molecular biology. Xylene Cyanol FF is characterized by its dark green or blue-grey crystalline appearance and has been found to have good light fastness, soaping, perspiration fastness, and oxygen bleaching properties.

Uses

Used in Molecular Biology:
Xylene Cyanol FF is used as a tracking dye for DNA sequencing, allowing researchers to monitor the progress of DNA molecules as they move through agarose or polyacrylamide gels during electrophoresis. The dye typically migrates with the DNA molecules around the 5kb size, providing a visual reference for the user.
Used in Electrophoresis:
In addition to DNA sequencing, Xylene Cyanol FF is also used in both electrophoresis polyacrylamide and agarose gels for various evaluations, including mobility shift analysis of single-stranded DNA (ssDNA).
Used in Spectrophotometric Assays:
Two highly sensitive spectrophotometric assays have been developed using Xylene Cyanol FF for the detection of platinum (IV) and chromium. These assays provide a means to accurately measure the concentration of these elements in samples.
Used in Vaccine Development:
As an organic dye, Xylene Cyanol FF has been utilized in the inactivation of the Sendai virus for vaccine development, offering strong protection against the virus.
Used in Textile Industry:
Xylene Cyanol FF's properties, such as light fastness, soaping, perspiration fastness, and oxygen bleaching, make it suitable for use in the textile industry as a dye for fabrics. Its fastness properties ensure that the color remains vibrant and resistant to various conditions, such as exposure to light, water, and sweat.

Preparation

4-Formylbenzene-1,3-disulfonic acid (1 Moore) and N-ethyl-2-methylbenzenamine (2 Moore) condensation, garnish with heavy chromate salt-oxalic acid oxidation its product.

Biochem/physiol Actions

Xylene Cyanol FF acts as nucleic acid stain.

Standard

Light Fastness

Fading

Stain

ISO

2

AATCC

2

InChI:InChI=1/C25H28N2O6S2.Na/c1-5-26-22-11-7-18(13-16(22)3)25(19-8-12-23(27-6-2)17(4)14-19)21-10-9-20(34(28,29)30)15-24(21)35(31,32)33;/h7-15,26H,5-6H2,1-4H3,(H,28,29,30)(H,31,32,33);/q;+1/p-1/b25-19-,27-23-;