295801-33-1Relevant articles and documents
Synthesis and Characterization of Fluorenone-, Anthraquinone-, and Phenothiazine-Labeled Oligodeoxynucleotides: 5′-Probes for DNA Redox Chemistry
Tierney, Mark T.,Grinstaff, Mark W.
, p. 5355 - 5359 (2007/10/03)
A facile and automated procedure for the synthesis of oligodeoxynucleotides possessing derivatives of 9-fluoreneone, 9,10-anthraquinone, and phenothiazine is described. The phosphoramidite approach is used to attach these redox and spectroscopic probes to the 5′-terminus of oligodeoxynucleotides in high yield (>95%). Thermal denaturation studies of labeled oligodeoxynucleotides show a slight enhancement in duplex stability relative to the unlabeled control, and circular dichroism spectra confirm B-form helical DNA structure in solution.