34725-61-6

Basic information

• Product Name: BROMOPHENOL BLUE SODIUM SALT
• Synonyms: LABOTEST-BB LT00159731;BROMOPHENOL BLUE, WATER SOLUBLE;BROMOPHENOL BLUE NA-SALT;BROMPHENOL BLUE;BROMPHENOL BLUE, SODIUM SALT;BROMPHENOL BLUE SODIUM SALT, WATER SOLUBLE;3',3'',5',5''-TETRABROMOPHENOLSULFONPHTHALEIN SODIUM SALT;3',3'',5',5''-TETRABROMOPHENOLSULFONEPHTHALEIN SODIUM SALT
• CAS NO: 34725-61-6
• Molecular Formula: C19H9Br4NaO5S
• Molecular Weight: 691.94
• EINECS: 263-653-2
• Product Categories: Biochemistry;Reagents for Electrophoresis;Analytical Chemistry;Indicator (pH);pH Indicators
• Mol File: 34725-61-6.mol
• Article Data: 0

Chemical Properties

• Melting Point: 300 °C
• Boiling Point: 688 °C at 760 mmHg
• Flash Point: 369.9 °C
• Appearance: blue, at pH above 4.6/
• Density: 0.985 g/mL at 25 °C
• Storage Temp.: room temp
• Water Solubility: Soluble in water.
• Merck: 14,1444
• BRN: 6995701
• CAS DataBase Reference: BROMOPHENOL BLUE SODIUM SALT(CAS DataBase Reference)
• NIST Chemistry Reference: BROMOPHENOL BLUE SODIUM SALT(34725-61-6)
• EPA Substance Registry System: BROMOPHENOL BLUE SODIUM SALT(34725-61-6)

Safety Data

• Hazard Codes: Xn
• Statements: 20/21/22-36/37/38
• Safety Statements: 24/25-22-36-26
• WGK Germany: 3
• TSCA: Yes
• Hazardous Substances Data: 34725-61-6(Hazardous Substances Data)

Usage

Description

Bromophenol Blue Sodium Salt, also known as Bromophenol Blue, is a tracking dye used in various laboratory applications. It is a purple liquid that aids in the visualization and analysis of proteins and nucleic acids during electrophoresis. Its chemical properties make it a valuable tool in the field of molecular biology and biochemistry.

Uses

Used in Molecular Biology and Biochemistry:
Bromophenol Blue Sodium Salt is used as a tracking dye for protein and nucleic acid electrophoresis in polyacrylamide gels. It helps researchers monitor the progress of the electrophoresis process and estimate the migration distance of the samples.
Used in Western Blotting Analysis:
Bromophenol Blue Sodium Salt is used in the preparation of protein samples for western blotting analysis. It aids in the visualization of protein bands on the membrane, allowing for accurate analysis and quantification of the proteins of interest.
Used in Drosophila Fly Food:
In the field of genetics and developmental biology, Bromophenol Blue Sodium Salt has been used in Drosophila fly food to aid visualization of the gut for dissection. This application helps researchers study the development and function of the fly's digestive system.
Used in Cell Viability Assays:
Bromophenol Blue Sodium Salt has been used in the buffer solution to differentiate dead and living cells. This application is crucial in cell biology research, where understanding cell viability is essential for various experiments and assays.
Used in Gel Loading Buffer:
Bromophenol Blue Sodium Salt is also used as a constituent in gel loading buffer. It helps in the visualization of the DNA or protein bands during agarose or polyacrylamide gel electrophoresis, allowing for accurate analysis and comparison of the samples.
Used in Histone Staining:
In the field of epigenetics and chromatin biology, Bromophenol Blue Sodium Salt has been used for staining histones. This application aids researchers in studying the structure and function of chromatin and the role of histones in gene regulation.

InChI:InChI=1/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1/rC19H9Br4NaO5S/c20-12-5-9(6-13(21)18(12)25)17(10-7-14(22)19(26)15(23)8-10)11-3-1-2-4-16(11)30(27,28)29-24/h1-8,25H