3625-57-8 Usage
Description
CI 51180, also known as Nile Blue A, is a stain used for discriminating between melanins and lipofuscins in paraffin sections of animal tissue. It is also useful for staining fats and phospholipids, as well as dyes and metabolites.
Uses
Used in Histology:
CI 51180 is used as a stain for discriminating between melanins and lipofuscins in paraffin sections of animal tissue. This helps in the identification and differentiation of these pigments, which can be crucial for diagnostic purposes.
Used in the Lillie and Fullmer Method:
CI 51180 is used in the Lillie and Fullmer method for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue. This method is particularly useful for studying the distribution and characteristics of these pigments in various tissues.
Used in Histochemical Analysis of Lipids:
CI 51180 is suitable for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue, and in a histochemical method for lipids in frozen sections. This allows for the visualization and analysis of lipids in tissue samples, which can be important for understanding various biological processes and conditions.
Biochem/physiol Actions
Nile blue A stains acidic components, such as phospholipids, nucleic and fatty acids by imparting a dark blue color, while neutral lipids, namely triglycerides, are stained pink, or red.
Purification Methods
Crystallise the dye from aqueous AcOH. It has UV: at 630nm (96% aqueous max EtOH) and 635nm (H2O). The betaine has UV: at 513nm (EtOH). [Crossley et al. J Am Chem Soc 74 max 57, 578 1952, Merill & Spencer J Am Chem Soc 70 3683 1948, Beilstein 27 II 457, 27 III/IV 5166.]
Check Digit Verification of cas no
The CAS Registry Mumber 3625-57-8 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 3,6,2 and 5 respectively; the second part has 2 digits, 5 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 3625-57:
(6*3)+(5*6)+(4*2)+(3*5)+(2*5)+(1*7)=88
88 % 10 = 8
So 3625-57-8 is a valid CAS Registry Number.
InChI:InChI=1/C20H19N3O.H2O4S/c1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;1-5(2,3)4/h5-12,21H,3-4H2,1-2H3;(H2,1,2,3,4)/p+1
