517-28-2 Usage
Description
Hematoxylin is a white to yellowish crystalline substance that redden upon exposure to light. It is a natural dye and a biological stain, commonly used in various applications across different industries.
Uses
Used in Ink Industry:
Hematoxylin is used as a colorant in the ink industry, providing a distinctive color to the inks for various purposes.
Used in Histology:
Hematoxylin is used as a biological stain in histology, specifically in the Hematoxylin and eosin (H&E) stain. This stain is one of the most commonly used in the field, allowing for the examination of cell nuclei under a microscope. Hematoxylin forms metal-haematein complexes that stain cell nuclei, aiding in the identification of basophilic structures, even though the staining mechanism differs from that of basic dyes.
Air & Water Reactions
Soluble in hot water, slightly water soluble in cold .
Reactivity Profile
Sensitive to light. .
Hazard
May be carcinogenic.
Purification Methods
Hematoxylin recrystallises from H2O (as trihydrate) in white-yellow crystals which become red on exposure to light and then melt at 100-120o. It has been recrystallised from Me2CO/*C6H6. It has been recrystallised as well from dilute aqueous NaHSO3 until colourless and is soluble in alkali, borax and glycerol. Store it in the dark below 0o. [Morsingh & Robinson Tetrahedron 26 182 1970, Dann & Hofmann Chem Ber 98 1498 1955, Beilstein 17/8 V 469.]
Check Digit Verification of cas no
The CAS Registry Mumber 517-28-2 includes 6 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 3 digits, 5,1 and 7 respectively; the second part has 2 digits, 2 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 517-28:
(5*5)+(4*1)+(3*7)+(2*2)+(1*8)=62
62 % 10 = 2
So 517-28-2 is a valid CAS Registry Number.
InChI:InChI=1/C16H14O6/c17-10-2-1-8-13-9-4-12(19)11(18)3-7(9)5-16(13,21)6-22-15(8)14(10)20/h1-4,13,17-21H,5-6H2/t13-,16-/m1/s1
517-28-2Relevant articles and documents
The Reaction of Hematoxylin with Zirconyl Chloride Studied Spectrophotometrically
Roubani-Kalantzopoulou, Fani,Katsanos, Nicholas A.
, p. 165 - 182 (2007/10/02)
The reaction of hematoxylin with zirconyl chloride in acid solutions was studied spectrophotometrically.A strong absorption band with λmax = 525 nm is attributed to a loose complex formed between hemateine and zirconium(IV) ions.The experimental findings - especially the fact that the linear absorption coefficient increases with time, passes through a maximum and then declines exponentially - are consistent with a four step mechanism.Two of the steps are fast equilibria, while the other two are slow dissociation reactions, the second being an order of magnitude faster than the first.The rate constants for the slow steps and the equillibrium constants for the fast steps have been determined.From the variation of these constants with temperature the activation parameters of the slow reactions and thermodynamic data for the fast equilibria have been calculated.Also the molar absorption coefficient of the absorbing complex has been estimated.Possible analytical applications of the reactions studied are briefly discussed. - Keywords: Zirconyl chloride, Hematoxylin complex formation rates, Activation parameters of complexes, Thermodynamic data of complexes
