55750-61-3 Usage
Description
N-Succinimidyl maleimidoacetate, also known as maleimidoacetic acid N-hydroxysuccinimide ester, is a hetero-bifunctional crosslinking reagent that contains NHS esterand maleimide-reactive groups at the opposite ends of a 4.4 ? spacer arm. This unique configuration allows for sequential, two-stage conjugation with amine and sulfhydryl functional groups, making it a versatile tool in the preparation of protein-hapten or protein-protein conjugates.
Uses
Used in Bioconjugation Chemistry:
N-Succinimidyl maleimidoacetate is used as a crosslinking reagent for the preparation of protein-protein or protein-hapten conjugates. Its ability to react with amine and sulfhydryl functional groups enables the formation of stable and specific conjugates, which are essential in various biological and medicinal applications.
Used in Pharmaceutical Industry:
In the pharmaceutical industry, N-Succinimidyl maleimidoacetate is used as a key component in the development of drug delivery systems and targeted therapies. Its crosslinking properties allow for the attachment of therapeutic agents to carrier molecules, such as antibodies or nanoparticles, improving the drug's efficacy and reducing side effects.
Used in Research and Development:
N-Succinimidyl maleimidoacetate is widely used in research and development for the study of protein interactions, structure, and function. Its ability to form stable conjugates between proteins and other molecules facilitates the investigation of protein-protein interactions, enzyme activity, and the development of novel bioassays.
Used in Diagnostics:
In the diagnostics field, N-Succinimidyl maleimidoacetate is employed for the development of immunoassays and biosensors. Its crosslinking capabilities enable the attachment of specific recognition elements, such as antibodies or aptamers, to detection platforms, enhancing the sensitivity and specificity of diagnostic tests.
Overall, N-Succinimidyl maleimidoacetate is a versatile and valuable reagent in various industries, including bioconjugation chemistry, pharmaceuticals, research and development, and diagnostics, due to its unique properties and ability to form stable conjugates with amine and sulfhydryl functional groups.
Check Digit Verification of cas no
The CAS Registry Mumber 55750-61-3 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 5,5,7,5 and 0 respectively; the second part has 2 digits, 6 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 55750-61:
(7*5)+(6*5)+(5*7)+(4*5)+(3*0)+(2*6)+(1*1)=133
133 % 10 = 3
So 55750-61-3 is a valid CAS Registry Number.
InChI:InChI=1/C10H8N2O6/c13-6-1-2-7(14)11(6)5-10(17)18-12-8(15)3-4-9(12)16/h1-2H,3-5H2
55750-61-3Relevant articles and documents
Synthesis N - maleic imide-based arylalkylic and its succinyl eater of method
-
, (2017/10/13)
The invention provides a method for synthesizing N-maleimidoalkyl acid and succinimido ester thereof, which comprises the following steps: (a) carrying out cyclization reaction on a compound disclosed as Formula (II) and phenyl 4-nitrotrifluoroacetate in an organic solvent in the presence of alkali to obtain N-maleimidoalkyl acid disclosed as Formula (III); and (b) reacting the N-maleimidoalkyl acid disclosed as Formula (III) and an acylation reagent in an organic solvent at reflux temperature to obtain an acyl chloride intermediate disclosed as (IV), reacting the acyl chloride intermediate disclosed as (IV) with N-hydroxy succinimide in an organic solvent in the presence of alkali to obtain the N-maleimidoalkyl acid succinimido ester disclosed as Formula (V). The method has the advantages of simple technique, high yield and high product purity, and is suitable for industrial production. The reaction route is disclosed in the specification.
Synthesis of N-Maleoyl-aminoacids and -peptides
Augustin, Manfred,Mueller, Wolfgang
, p. 789 - 798 (2007/10/02)
N-Maleoyl-aminoacid-N'-hydroxysuccinimidesters 3 or N-maleoyl-aminobenzoic acids 5 are synthsized from N-maleyl-aminoacids 1 on different ways.N-maleoyl-aminobenzoic-4-nirophenyl-, -2-nitro-phenyl- or 2,4-dinitro-phenylesters 6, 7 and 8 will be obtained from 1 or 5. o-Mercaptoaniline, thiourea or cysteine react with 5 to benzothiazines 9, thiazolidines 10 and 1,4-thiazines 11.From 5 the peptides 12 are yielded.The pentapeptide 13 are formed from 12 by addition of glutathione.
Preparation and some properties of maleimido acids and maleoyl derivatives of peptides.
Keller,Rudinger
, p. 531 - 541 (2007/10/05)
-
