58-68-4Relevant articles and documents
Mechanistic Aspects of the Electrochemical Oxidation of Dihydronicotinamide Adenine Dinucleotide (NADH)
Moiroux, Jacques,Elving, Philip J.
, p. 6533 - 6538 (1980)
The apparently single stage anodic oxidation of NADH involving removal of two electrons and a proton to form NAD+ has been examined with particular attention to the deprotonation step and its relationship to the initial potential-determining electron-transfer step, primarily at glassy carbon electrodes (GCE) in aqueous media with supplementary studies at pyrolytic graphite and platinum electrodes in aqueous media and at GCE in Me2SO; the carbon electrodes were generally first covered with an adsorbed NAD+ layer in order to eliminate adsorption-controlled faradaic processes.The initial step is an irreversible heterogeneous electron transfer (transfer coefficient β = 0.37 at carbon electrodes and 0.43 at platinum).The resulting cation radical NAD.H+ loses a proton (first-order reaction; rate constant k) to form the neutral radical NAD. which may participate in a second heterogeneous electron transfer (ECE mechanism) or in a homogeneous electron transfer with NAD.H+ (disproportionation mechanism DISP 1 or half-regeneration mechanism), yielding NAD+.The near identities of current functions, viscosity-corrected diffusion coefficients D and β values, point to essentially similar solute species and charge-transfer paths being involved in different media and at different electrodes.D is ca. 2 x 10-6 cm2 s-1 in aqueous solution; k is ca. 60 s-1 at the GCE covered with adsorbed NAD+.
Chemo-bio catalysis using carbon supports: application in H2-driven cofactor recycling
Cleary, Sarah E.,Grobert, Nicole,Reeve, Holly A.,Vincent, Kylie A.,Zhao, Xu,Zor, Ceren
, p. 8105 - 8114 (2021/06/22)
Heterogeneous biocatalytic hydrogenation is an attractive strategy for clean, enantioselective CX reduction. This approach relies on enzymes powered by H2-driven NADH recycling. Commercially available carbon-supported metal (metal/C) catalysts are investigated here for direct H2-driven NAD+reduction. Selected metal/C catalysts are then used for H2oxidation with electrons transferredviathe conductive carbon support material to an adsorbed enzyme for NAD+reduction. These chemo-bio catalysts show improved activity and selectivity for generating bioactive NADH under ambient reaction conditions compared to metal/C catalysts. The metal/C catalysts and carbon support materials (all activated carbon or carbon black) are characterised to probe which properties potentially influence catalyst activity. The optimised chemo-bio catalysts are then used to supply NADH to an alcohol dehydrogenase for enantioselective (>99% ee) ketone reductions, leading to high cofactor turnover numbers and Pd and NAD+reductase activities of 441 h?1and 2347 h?1, respectively. This method demonstrates a new way of combining chemo- and biocatalysis on carbon supports, highlighted here for selective hydrogenation reactions.
Highly Efficient S-g-CN/Mo-368 Catalyst for Synergistically NADH Regeneration Under Solar Light
Gupta, Abhishek Kumar,Gupta, Sarvesh Kumar,Singh, Ajeet,Yadav, Bal Chandra,Yadav, Rajesh Kumar
, (2021/07/31)
Sulfur-doped graphitic carbon nitride (S-g-CN) has gained significant attention in recent years. Sulfur-doped graphitic carbon nitride (S-g-CN) is a promising metal-free photocatalyst because of its band orientation, natural abundance and groundwork. Improved photocatalytic activity of S-g-CN material for solar chemical production persists a hot yet challenging problem. Herein, we provide an adaptable method for the synthesis of S-g-CN nanocomposite decorated with the moiety of giant polyoxometalate (S-g-CN/Mo-368) that subsequently showed highly efficient photocatalytic activity. The as-synthesized S-g-CN/Mo-368 as a recyclable artificial photocatalyst revealed excellent activity for solar chemical production, that is nicotinamide adenine dinucleotide (NADH) regeneration under visible light. The immobilized Mo-368 on the S-g-CN surface increased the visible light adsorption capacity of the S-g-CN/Mo-368 photocatalyst. The visible light absorption activity, morphology, element compositions, particle size and zeta potential of S-g-CN powder and S-g-CN/Mo-368 were thoroughly investigated. From the application point of view, S-g-CN/Mo-368 was applied to determine the solar chemical production (i.e. NADH regeneration) under visible light with a higher yield% of about ~ 94.85%.
