591-28-6 Usage
Description
4-Thiouracil is a site-specific, photoactivatable probe used to detect RNA structures and nucleic acid-nucleic acid contacts. It absorbs ultraviolet light >300 nm and, in the presence of oxygen, acts as an energy donor to produce singlet oxygen by triplet-triplet energy transfer. The highly reactive oxygen species then reacts readily with 4-thiouracil, leading to the production of uracil and uracil-6-sulfonate, which is fluorescent at a wavelength of ~390 nm. 4-Thiouracil is also used as a T. gondii uracil phosphoribosyltransferase substrate to produce 4-thiouridine monophosphate, which can ultimately be incorporated into RNA.
Uses
Used in Molecular Biology Research:
4-Thiouracil is used as a reagent in Schneider's media for the embryos during RNA extraction. It is employed for tagging in cell type-specific RNA isolation from intact complex tissues, allowing for the detection and analysis of specific RNA structures and interactions.
Used in Northwestern Blotting Technique:
4-Thiouracil is used as a reagent for the Northwestern blotting technique, which is a method used to study protein-RNA interactions. Its photoactivatable properties enable the detection of specific RNA-protein interactions, providing valuable insights into the molecular mechanisms of gene regulation and other biological processes.
Check Digit Verification of cas no
The CAS Registry Mumber 591-28-6 includes 6 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 3 digits, 5,9 and 1 respectively; the second part has 2 digits, 2 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 591-28:
(5*5)+(4*9)+(3*1)+(2*2)+(1*8)=76
76 % 10 = 6
So 591-28-6 is a valid CAS Registry Number.
InChI:InChI=1/C4H4N2OS/c7-4-5-2-1-3(8)6-4/h1-2H,(H2,5,6,7,8)
591-28-6Relevant articles and documents
A one-pot, water compatible synthesis of pyrimidine nucleobases under plausible prebiotic conditions
Okamura, Hidenori,Becker, Sidney,Tiede, Niklas,Wiedemann, Stefan,Feldmann, Jonas,Carell, Thomas
supporting information, p. 1939 - 1942 (2019/05/02)
Herein, we report a new prebiotically plausible pathway towards a pyrimidine nucleobase in continuous manner. The route involves simultaneous methylation and carbamoylation of cyanoacetylene-derived α,β-unsaturated thioamide with N-methyl-N-nitrosourea (MNU) in aqueous media. This provides S-methylpyrimidinone in one-pot, which can be converted into a variety of 4-substituted pyrimidine nucleobases including cytosine and uracil.
FUSED HETEROARYL DERIVATIVES AS OREXIN RECEPTOR ANTAGONISTS
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Page/Page column 105, (2016/07/27)
Fused heteroaryl derivative compounds which are antagonists of orexin receptors are provided. The compounds can be used in the potential treatment or prevention of neurological and psychiatric disorders and diseases in which orexin receptors are involved. Also provided is a composition which comprises the compound can be use to prevent or treat such diseases in which orexin receptors are involved.
Artificial DNA base pair analogues
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, (2008/06/13)
The present invention is directed to new artificial base pairs comprising complementary artificial purines and pyrimidines and methods of using artificial complementary base pairs.