60129-60-4Relevant articles and documents
Further study on the 1,4-alpha-transglucosylation of rubusoside, a sweet steviol-bisglucoside from Rubus suavissimus.
Ohtani,Aikawa,Ishikawa,Kasai,Kitahata,Mizutani,Doi,Nakaura,Tanaka
, p. 449 - 453 (1991)
Rubusoside (the beta-D-glucosyl ester of 13-O-beta-D-glucosyl-steviol), which is the major sweet principle of leaves of Rubus suavissimus S. Lee, was subjected to 1,4-alpha-transglucosylation by the cyclodextringlucanotransferase-starch system (the CGTase system). The tri- and tetra-glucosylated products were isolated together with the mono- and di-glucosylated products, which had already been isolated. A prominent increase in intensity of the sweetness was observed for the compounds which were di- and tri-glucosylated at the 13-O-glucosyl moiety. This result further substantiated the structure-sweetness relationship for 1,4-alpha-glucosylated compounds of steviol-glycosides reported previously. For protection of the 19-COO-glucosyl moiety against glucosylation by the CGTase system, the 4-hydroxyl group of the 19-COO-glucosyl moiety was beta-galactosylated by the beta-galactosidase-lactose system. This galactosylated compound was subjected to a regio-selective glucosylation of the 13-O-glucosyl moiety by the CGTase system, which was followed by enzymic elimination of the galactosyl group to furnish an exclusive preparation of the improved sweeteners just mentioned.
Discovery of: Arabidopsis UGT73C1 as a steviol-catalyzing UDP-glycosyltransferase with chemical probes
Zhou, Yiqing,Li, Weichao,You, Wenjing,Di, Zhengao,Wang, Mingli,Zhou, Haiyan,Yuan, Shuguang,Wong, Nai-Kei,Xiao, Youli
supporting information, p. 7179 - 7182 (2018/07/03)
Chemoselective and affinity-based probes of steviol were applied to the discovery of glycosyltransferase activity and corresponding UDP-glycosyltransferase in Arabidopsis thaliana, which illustrates a simple strategy for rapidly harnessing minable novel biological parts from plants for synthetic biology.
PRODUCTION OF STEVIOL GLYCOSIDES IN RECOMBINANT HOSTS
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Paragraph 0052; 00202, (2016/08/23)
The invention relates to recombinant microorganisms and methods for producing steviol glycosides, glycosylated ent-kaurenol, and glycosylated ent-kaurenoic acid.