60311-02-6

Basic information

• Product Name: SULFORHODAMINE 101
• Synonyms: CI 45100;8-(2,4-DISULFOPHENYL)-2,3,5,6,11,12,14,15-OCTAHYDRO-1H,4H,10H,13H-DIQUINOLIZINO[9,9A,1-BC:9',9A',1-HI]XANTHENE;SULFORHODAMINE 640;Sulforhodamine 101 (free acid);SULFORHODAMINE 101 ACID;SULFORHODAMINE;SULFORHODAMINE 101;SR101
• CAS NO: 60311-02-6
• Molecular Formula: C₃₁H₃₀N₂O₇S₂
• Molecular Weight: 606.71
• EINECS: 262-159-4
• Product Categories: S;Stains and Dyes;Stains&Dyes, A to
• Mol File: 60311-02-6.mol
• Article Data: 6

Chemical Properties

• Appearance: green to dark green crystalline powder
• Density: 1.1982 (rough estimate)
• Refractive Index: 1.6900 (estimate)
• Storage Temp.: Inert atmosphere,Room Temperature
• Solubility: methanol: soluble1mg/mL
• BRN: 3582548
• CAS DataBase Reference: SULFORHODAMINE 101(CAS DataBase Reference)
• NIST Chemistry Reference: SULFORHODAMINE 101(60311-02-6)
• EPA Substance Registry System: SULFORHODAMINE 101(60311-02-6)

Safety Data

• Safety Statements: 22-24/25
• WGK Germany: 3
• F: 8
• Hazardous Substances Data: 60311-02-6(Hazardous Substances Data)

Usage

Description

SULFORHODAMINE 101 is a nonfixable red fluorescent dye, characterized by its green to dark green crystalline powder form. It has an excitation maximum at 586 nm and an emission maximum at 605 nm. This dye is known for its ability to serve as a specific marker for astrocytes and as an activity-dependent probe for monitoring regulated exocytosis. Additionally, it has been reported to induce long-term potentiation of intrinsic neuronal excitability and a long-lasting increase in evoked excitatory postsynaptic potentials in CA1 pyramidal neurons in hippocampal slices.

Uses

Used in Neurophysiological Applications:
SULFORHODAMINE 101 is used as a red fluorescent dye for calcium imaging methods in neurophysiological experiments. This allows researchers to study the dynamics of calcium ions, which play a crucial role in various cellular processes, including neurotransmission and synaptic plasticity.
Used in Astrocyte Labeling:
SULFORHODAMINE 101 is used as a red-emitting fluorophore to label astrocytes, which are star-shaped glial cells in the central nervous system. Labeling these cells helps researchers to better understand their function and interactions with other cells in the brain.
Used in Dyes and Metabolites:
SULFORHODAMINE 101 is also utilized in the study of dyes and metabolites, where its red fluorescence properties can be advantageous for various experimental setups and analyses.

Downstream Products

• 541-59-3 maleiimide 
• 1590395-70-2 C₃₉H₄₅N₇O₉S₂ 

Relevant articles and documents

Substractive single label comparative hybridization

Provided are methods of determining differences between nucleic acids in a test sample and a reference sample. In certain embodiments the methods are used for detecting and mapping chromosomal or genetic abnormalities associated with various diseases or with predisposition to various diseases, or to detecting the phenomena of large scale copy number variants. In particular, provided are advanced methods of performing array-based comparative hybridization that allow reproducibility between samples and enhanced sensitivity by using the same detectable label for both test sample and reference sample nucleic acids. Invention methods are useful for the detection or diagnosis of particular disease conditions such as cancer, and detecting predisposition to cancer based on detection of chromosomal or genetic abnormalities and gene expression level. Invention methods are also useful for the detection or diagnosis of hereditary genetic disorders or predisposition thereto, especially in prenatal samples. Moreover, invention methods are also useful for the detection or diagnosis of de novo genetic aberrations associated with post-natal developmental abnormalities.

Oligonucleotides comprising a molecular switch

This invention relates to oligonucleotides comprising a molecular switch which may exist in an “open” or “closed” position. The molecular switch portion of the probe is particularly sensitive to the identity of sequences complementary to the molecular switch. Oligonucleotides containing a molecular switch are applicable to all kinds of hybridization processes. Due to the sensitivity of the switch domain of the oligonucleotide, probes containing a molecular switch are particularly useful in the identification of single point mismatches. More specifically, a portion, but not all, of the oligonucleotide becomes unbound from a mismatched target. The invention further relates to methods of using said oligonucleotides for research reagents, and clinical diagnostics. An exemplary oligonucleotide comprises a first hybridizable domain, a second bridging block domain, and a third binding domain.

Dual resonance energy transfer nucleic acid probes

Dual nucleic acid probes with resonance energy transfer moieties are provided. In particular, fluorescent or luminescent resonance energy transfer moieties are provided on hairpin stem-loop molecular beacon probes that hybridize sufficiently near each other on a subject nucleic acid, e.g. mRNA, to generate an observable interaction. The invention also provides lanthanide chelate luminescent resonance energy transfer moieties on linear and stem-loop probes that hybridize sufficiently near each other on a subject nucleic acid to generate an observable interaction. The invention thereby provides detectable signals for rapid, specific and sensitive hybridization determination in vivo. The probes are used in methods of detection of nucleic acid target hybridization for the identification and quantification of tissue and cell-specific gene expression levels, including response to external stimuli, such as drug candidates, and genetic variations associated with disease, such as cancer.