6050-81-3 Usage
Description
Acetylthiocholine chloride is a synthetic compound that serves as a substrate for acetylcholinesterase, an enzyme involved in the hydrolysis of acetylcholine. It is structurally similar to acetylcholine, with a thiol group replacing the hydroxyl group, which allows it to be used in various assays and studies related to acetylcholinesterase activity.
Uses
Used in Biochemical Research:
Acetylthiocholine chloride is used as a substrate for acetylcholinesterase in biochemical research to study the enzyme's kinetic parameters and activity. It is particularly useful in microcalorimetric studies, where it helps in determining the thermodynamic properties and reaction rates of acetylcholinesterase.
Used in Medical Research:
Acetylthiocholine chloride is used in medical research to determine the acetylcholine esterase activity of semen exosomes (SE). This application aids in understanding the role of acetylcholinesterase in male fertility and the potential use of semen exosomes as biomarkers for fertility assessment.
Biochem/physiol Actions
Acetylcholinesterase (AChE) enzyme plays an important physiological role in the neurotransmission process.
Purification Methods
The chloride can be purified in the same way as the bromide, and it can be prepared from the iodide. A few milligrams dissolved in H2O can be purified by applying onto a Dowex-1 Cl-resin column (prepared by washing with N HCl followed by CO3 -free H2O until the pH is 5.8). After equilibration for 10minutes, elution is started with CO3 -free distilled H2O, and 3mL fractions are collected and their OD values at 229nm are measured. The fractions with appreciable absorption are pooled and lyophilised at 0-5o. Note that at higher temperatures decomposition of the ester is appreciable; hydrolysis is appreciable at pH >10.5/20o. The residue is dried in vacuo over P2O5, checked for traces of iodine (add conc H2SO4 and heat, violet vapours are released), and recrystallise it from propan-1-ol. [Gal & Roth Clin Chim Acta 2 316 1957, Beilstein 4 IV 1585.]
Check Digit Verification of cas no
The CAS Registry Mumber 6050-81-3 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 6,0,5 and 0 respectively; the second part has 2 digits, 8 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 6050-81:
(6*6)+(5*0)+(4*5)+(3*0)+(2*8)+(1*1)=73
73 % 10 = 3
So 6050-81-3 is a valid CAS Registry Number.
InChI:InChI=1/C7H16NOS.ClH/c1-7(9)10-6-5-8(2,3)4;/h5-6H2,1-4H3;1H/q+1;/p-1
