6243-71-6Relevant articles and documents
A carbazole-hemicyanine dye based ratiometric fluorescent probe for selective detection of bisulfite (HSO3 ?) in cells and C. elegans
Bi, Keying,Tan, Rui,Hao, Ruiting,Miao, Lanxi,He, Yanqi,Xianghua, Wu,Zhang, Junfeng,Rui, Xu
supporting information, p. 545 - 548 (2018/12/11)
Bisulfite (HSO3 ?) is an important sulfur dioxide (SO2) derivative, which plays a major role in many physiological processes and is also closely associated with a variety of diseases. Thus the development of highly selective and sensitive fluorescent probes is essential to detect HSO3ˉ in living cells. In this work, we report the synthesis and analysis of a ratiometric fluorescent probe for selective detection of HSO3ˉ based on the 1,4-nucleophilic addition reaction with the carbazole as an electron donor (D) and aldehyde group as an electron acceptor (A). The addition of HSO3ˉ and other ions to our probe can be observed by UV - vis and fluorescence spectrometry. Our investigation proved that the probe is highly selective and sensitive for HSO3ˉ and ratiometric changes. Moreover, the probe has good cell permeability and was successfully applied to the detection of exogenous HSO3ˉ in Hela cells and C. elegans.
FRET-Based Mito-Specific Fluorescent Probe for Ratiometric Detection and Imaging of Endogenous Peroxynitrite: Dyad of Cy3 and Cy5
Jia, Xiaotong,Chen, Qiangqiang,Yang, Yingfang,Tang, Yao,Wang, Rui,Xu, Yufang,Zhu, Weiping,Qian, Xuhong
supporting information, p. 10778 - 10781 (2016/09/09)
Peroxynitrite (OONO-) is profoundly implicated in health and disease. The physiological and pathological outcome of OONO- is related to its local concentration, and hence, a reliable OONO- assay is highly desired. We have developed a FRET-based small-molecule fluorescent probe (PNCy3Cy5), harnessing the differential reactivity of Cy3 and Cy5 toward OONO- by fine-tuning. It exhibits high detection sensitivity and yields a ratiometric fluorescent signal. We have exemplified that it can be applied in semiquantitative determination of OONO- in living cells. Notably, it specifically localizes in mitochondria, where endogenous OONO- is predominantly generated. Thus, PNCy3Cy5 is a promising molecular tool for peroxynitrite biology.
Highly selective visual detection of Cu(II) utilizing intramolecular hydrogen bond-stabilized merocyanine in aqueous buffer solution
Guo, Zhao-Qi,Chen, Wei-Qiang,Duan, Xuan-Ming
supporting information; experimental part, p. 2202 - 2205 (2010/08/06)
A Cu2+-specific colorimetric sensor 1, which is stabilized by an intramolecular hydrogen bonding, was designed and developed. The color of 1 changes from purple to blue on addition of 1.0 μM Cu2+ in aqueous buffer solution, which can be detected by the naked eye. The analytical detection limit for Cu2+ by the naked eye is as low as 1.0 μM. The stoichiometry for 1 and Cu2+ in complex is 2:1 in aqueous solution.