71484-85-0 Usage
Description
4-Nitrophenyl alpha-D-Glucuronide is a chemical compound that serves as a substrate for beta-glucuronidase enzyme in biochemical research and pharmaceutical applications. It is instrumental in assays to detect and quantitate enzyme activity, as well as in studying the metabolic pathway of glucuronidation, a crucial phase II detoxification process. This colorless to light yellow powder is stable under normal temperatures and pressures and plays a significant role in drug metabolism and toxicology for understanding conjugation and detoxification processes in the body.
Uses
Used in Biochemical Research:
4-Nitrophenyl alpha-D-Glucuronide is used as a substrate for beta-glucuronidase enzyme assays to detect and quantitate the enzyme's activity. This is essential for understanding the enzyme's role in various biological processes.
Used in Pharmaceutical Applications:
In the pharmaceutical industry, 4-Nitrophenyl alpha-D-Glucuronide is used to study the metabolic pathway of glucuronidation, an important phase II detoxification process. This helps in the development of drugs and understanding their metabolism and potential toxicity.
Used in Drug Metabolism and Toxicology:
4-Nitrophenyl alpha-D-Glucuronide is used as a tool to investigate the conjugation and detoxification processes in the body. This is vital for assessing the safety and efficacy of drugs and their potential side effects.
Used in the Study of Metabolic Pathways:
4-Nitrophenyl alpha-D-Glucuronide is employed to explore the glucuronidation pathway, which is a key component of the body's detoxification system. Understanding this pathway is crucial for developing treatments and managing diseases related to detoxification processes.
Check Digit Verification of cas no
The CAS Registry Mumber 71484-85-0 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 7,1,4,8 and 4 respectively; the second part has 2 digits, 8 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 71484-85:
(7*7)+(6*1)+(5*4)+(4*8)+(3*4)+(2*8)+(1*5)=140
140 % 10 = 0
So 71484-85-0 is a valid CAS Registry Number.
InChI:InChI=1/C12H13NO9/c14-7-8(15)10(11(17)18)22-12(9(7)16)21-6-3-1-5(2-4-6)13(19)20/h1-4,7-10,12,14-16H,(H,17,18)/t7-,8-,9-,10+,12-/m0/s1
71484-85-0Relevant articles and documents
DNA-catalyzed glycosylation using aryl glycoside donors
Hesser, Anthony R.,Brandsen, Benjamin M.,Walsh, Shannon M.,Wang, Puzhou,Silverman, Scott K.
supporting information, p. 9259 - 9262 (2016/07/25)
We report the identification by in vitro selection of Zn2+/Mn2+-dependent deoxyribozymes that glycosylate the 3′-OH of a DNA oligonucleotide. Both β and α anomers of aryl glycosides can be used as the glycosyl donors. Individual deox
Enzymic synthesis of β-glucuronides of estradiol, ethynylestradiol and other phenolic substrates on a preparative scale employing UDP-glucuronyl transferase
Werschkun, Barbara,Wendt, Andreas,Thiem, Joachim
, p. 3021 - 3023 (2007/10/03)
By incubation of bovine liver UDP-glucuronyl transferase with various phenolic aglycone substrates, preparation of the corresponding β-glucuronides could be achieved in milligram quantities. Concomitantly, simple access was generated to estrogen steroid glucuronides of great biological importance. In terms of experimental feasibility, direct application of the commercially available crude microsomal product was compared to and proven to be competitive with the usually preferred utilization of an immobilized enzyme preparation.
