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72-57-1

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  • 2,7-Naphthalenedisulfonicacid,3,3'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(2,1-diazenediyl)]bis[5-amino-4-hydroxy-,sodium salt (1:4) 72-57-1

    Cas No: 72-57-1

  • No Data

  • 1 Kilogram

  • 10000 Metric Ton/Month

  • Shanghai Upbio Tech Co.,Ltd
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72-57-1 Usage

Description

Direct Blue 14 is an azo-based, hydrophilic, tetrasulfonated blue acid dye that is widely used as a biological stain and for assessing cell viability.

Uses

Used in Biological Staining:
Direct Blue 14 is used as a biological stain for various applications, including staining tissues and cells for microscopic examination.
Used in Cell Viability Assessment:
Direct Blue 14 is used as a cell viability indicator in the Trypan Blue exclusion assay. It stains dead cells with permeable membranes blue, while living cells with intact membranes exclude the dye. This allows for the visual determination of living versus dead cells, making it an easy method for assessing cell viability in cultured cells and other samples such as rat abdominal organ slices and islet cell clusters from caprine pancreatic samples.

Preparation

3,3′-Dimethylbenzidine double nitriding, in alkaline conditions and 4-Amino-5-hydroxynaphthalene-2,7-disulfonic acid(2 Moore) coupled.

Reactivity Profile

Direct Blue 14 is an azo compound. Azo, diazo, azido compounds can detonate. This applies in particular to organic azides that have been sensitized by the addition of metal salts or strong acids. Toxic gases are formed by mixing materials of this class with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acyl halides, and strong oxidizing or reducing agents. Flammable gases are formed by mixing materials in this group with alkali metals. Explosive combination can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. Direct Blue 14 is incompatible with strong oxidizing agents and strong acids.

Health Hazard

ACUTE/CHRONIC HAZARDS: Direct Blue 14 may cause irritation and may be absorbed through the skin. It is a positive animal carcinogen. When heated to decomposition Direct Blue 14 emits very toxic fumes of nitrogen oxides, sulfur oxides, carbon monoxide and carbon dioxide.

Fire Hazard

Flash point data for Direct Blue 14 are not available; however, Direct Blue 14 is probably combustible.

Biochem/physiol Actions

Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.

Safety Profile

Suspected carcinogen with experimental carcinogenic, neoplastigenic, and tumorigenic data. Poison by intraperitoneal, intravenous, and subcutaneous routes. Experimental teratogenic and reproductive effects. Mutation data reported. When heated to decomposition it emits very toxic fumes of NOx NazO, and SOx

Potential Exposure

Used in dyeing textiles; leather and paper; as a biological stain.

Standard

Acid Resistance

Fading

Stain

ISO

4-5

AATCC

5

Incompatibilities

Dust may form explosive mixture with air. Trypan Blue is incompatible with strong oxidizing agents. Incompatible with oxidizers (chlorates, nitrates, peroxides, permanganates, perchlorates, chlorine, bromine, fluorine, etc.); contact may cause fires or explosions. Keep away from alkaline materials, strong bases, strong acids, oxoacids, epoxides. Azo compounds can detonate. This applies in particular to organic azides that have been sensitized by the addition of metal salts or strong acids. Toxic gases are formed by mixing materials of this class with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acylhalides, and strong oxidizing or reducing agents. Flammable gases are formed by mixing materials in this group with alkali metals. Explosive combinations can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. Trypan Blue is sensitive to prolonged exposure to heat.

Check Digit Verification of cas no

The CAS Registry Mumber 72-57-1 includes 5 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 2 digits, 7 and 2 respectively; the second part has 2 digits, 5 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 72-57:
(4*7)+(3*2)+(2*5)+(1*7)=51
51 % 10 = 1
So 72-57-1 is a valid CAS Registry Number.
InChI:InChI=1/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,37-38H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4/b39-31+,40-32+;;;;

72-57-1 Well-known Company Product Price

  • Brand
  • (Code)Product description
  • CAS number
  • Packaging
  • Price
  • Detail
  • TCI America

  • (T0556)  Direct Blue 14  

  • 72-57-1

  • 25g

  • 430.00CNY

  • Detail
  • Alfa Aesar

  • (A18600)  Trypan Blue, dye content >60%   

  • 72-57-1

  • 25g

  • 331.0CNY

  • Detail
  • Alfa Aesar

  • (A18600)  Trypan Blue, dye content >60%   

  • 72-57-1

  • 100g

  • 963.0CNY

  • Detail
  • Alfa Aesar

  • (A18600)  Trypan Blue, dye content >60%   

  • 72-57-1

  • 500g

  • 3818.0CNY

  • Detail
  • Sigma-Aldrich

  • (93595)  TrypanBluesolution  0.4%, for microscopy

  • 72-57-1

  • 93595-50ML

  • 486.72CNY

  • Detail
  • Sigma-Aldrich

  • (93595)  TrypanBluesolution  0.4%, for microscopy

  • 72-57-1

  • 93595-250ML

  • 987.48CNY

  • Detail

72-57-1SDS

SAFETY DATA SHEETS

According to Globally Harmonized System of Classification and Labelling of Chemicals (GHS) - Sixth revised edition

Version: 1.0

Creation Date: Aug 12, 2017

Revision Date: Aug 12, 2017

1.Identification

1.1 GHS Product identifier

Product name trypan blue

1.2 Other means of identification

Product number -
Other names congoblue

1.3 Recommended use of the chemical and restrictions on use

Identified uses For industry use only.
Uses advised against no data available

1.4 Supplier's details

1.5 Emergency phone number

Emergency phone number -
Service hours Monday to Friday, 9am-5pm (Standard time zone: UTC/GMT +8 hours).

More Details:72-57-1 SDS

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