73463-39-5 Usage
Description
3-(Cyclohexylamino)-2-hydroxy-1-propanesulfonic acid, also known as CAPSO, is a biological and zwitterionic buffer commonly categorized as a Good's buffer. It is a white powder with a second dissociation constant pK2 value that has been measured. CAPSO has a useful pH range of 8.9 10.3 and is known for its minimal reactivity with enzymes or proteins and minimal salt effects.
Uses
Used in Biological Applications:
3-(Cyclohexylamino)-2-hydroxy-1-propanesulfonic acid is used as a biological buffer for various applications, including:
1. Maintaining pH 9 during water leach test for the analysis of high-carbon coal fly ash-soil mixtures.
2. Chemiluminescent in situ hybridization using soybean peroxidase-labeled peptide nucleic acid (PNA) probes.
3. Preparation of planar bilayer lipid membranes.
Used in Electrophoresis:
3-(Cyclohexylamino)-2-hydroxy-1-propanesulfonic acid is used as a buffer for protein separations and diagnostic reagent production in electrophoretic analyses.
Used in Electrotransfer of Proteins:
3-(Cyclohexylamino)-2-hydroxy-1-propanesulfonic acid is used in the electrotransfer of proteins to PVDF or nitrocellulose membranes, where its low metal-binding constants make it particularly suited for investigating metal-dependent enzymes.
Used in Immunoblotting and Westerns:
3-(Cyclohexylamino)-2-hydroxy-1-propanesulfonic acid is utilized as a buffer in immunoblotting and western blotting techniques, providing optimal buffering at pH 10.4.
Check Digit Verification of cas no
The CAS Registry Mumber 73463-39-5 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 7,3,4,6 and 3 respectively; the second part has 2 digits, 3 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 73463-39:
(7*7)+(6*3)+(5*4)+(4*6)+(3*3)+(2*3)+(1*9)=135
135 % 10 = 5
So 73463-39-5 is a valid CAS Registry Number.
InChI:InChI=1/C9H19NO4S/c11-9(7-15(12,13)14)6-10-8-4-2-1-3-5-8/h8-11H,1-7H2,(H,12,13,14)/t9-/m1/s1