74299-50-6

Basic information

• Product Name: 2'-DEOXYADENOSINE-5'-TRIPHOSPHATE DISODIUM SALT
• Synonyms: 2'-deoxyadenosine 5'-triphosphate disodiuM sal;Adenosine5'-(tetrahydrogen triphosphate), 2'-deoxy-, disodiuM salt (9CI);2'-Deoxyadenosine 5'-triphosphate sodium salt (dATP);Sodium ((2R,3S,5R)-5-(6-amino-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl)methyl dihydrogentriphosphate;2'-DEOXY-ADENOSIN-5'-TRIPHOSPHATE TETRASODIUM SALT;2'-DEOXYADENOSINE 5'-TRIPHOSPHORIC ACID;2'-DEOXYADENOSINE-5'-TRIPHOSPHORIC ACID, DISODIUM;2'-DEOXYADENOSINE 5'-TRIPHOSPHORIC ACID DISODIUM SALT
• CAS NO: 74299-50-6
• Molecular Formula: C10H14N5Na2O12P3
• Molecular Weight: 535.15
• EINECS: 277-809-2
• Product Categories: nucleoside
• Mol File: 74299-50-6.mol
• Article Data: 0

Chemical Properties

• Appearance: White/Powder
• Storage Temp.: −20°C
• Water Solubility: Soluble in water.
• CAS DataBase Reference: 2'-DEOXYADENOSINE-5'-TRIPHOSPHATE DISODIUM SALT(CAS DataBase Reference)
• NIST Chemistry Reference: 2'-DEOXYADENOSINE-5'-TRIPHOSPHATE DISODIUM SALT(74299-50-6)
• EPA Substance Registry System: 2'-DEOXYADENOSINE-5'-TRIPHOSPHATE DISODIUM SALT(74299-50-6)

Safety Data

• Hazard Codes: Xn,Xi
• Statements: 20/21/22-36/37/38
• Safety Statements: 26-36-37/39-36/37/39
• WGK Germany: 3
• RTECS: AU7358800
• F: 10-21
• Hazardous Substances Data: 74299-50-6(Hazardous Substances Data)

Usage

Description

2'-Deoxyadenosine-5'-triphosphate disodium salt, also known as dATP, is a nucleotide that plays a crucial role in various biological processes. It is a deoxyribonucleotide triphosphate, which means it is a modified form of ATP lacking a 2'-hydroxyl group on the ribose sugar. This modification is essential for its function in DNA synthesis and other applications.

Uses

Used in Molecular Biology Research:
2'-Deoxyadenosine-5'-triphosphate disodium salt is used as a component in molecular biology research for various applications, including:
1. Smac-XIAP Binding Assay:
2'-Deoxyadenosine-5'-triphosphate disodium salt is used as a component in the Smac-X-linked inhibitor of apoptosis protein (Smac-XIAP) binding assay. This assay is crucial for studying the interaction between Smac and XIAP proteins, which play a role in regulating apoptosis (cell death).
2. Polymerase Chain Reaction (PCR):
dATP is used as a substrate in the polymerase chain reaction (PCR) process, specifically when using AM-toxin primers. PCR is a technique used to amplify specific DNA sequences, and dATP is one of the four nucleotides required for DNA synthesis during this process.
3. Determining Affinity Constants:
2'-Deoxyadenosine-5'-triphosphate disodium salt is used to determine the affinity constant (Kd) with the transcription termination factor Rho. This helps researchers understand the binding strength between Rho and its target molecules, which is essential for studying gene regulation and expression.
4. DNA Synthesis:
dATP is used as a substrate by a variety of polymerases, including DNA polymerases and reverse transcriptases. These enzymes are responsible for synthesizing DNA from a template strand, and dATP provides the necessary building blocks for this process.