82-76-8 Usage
Description
8-Anilino-1-naphthalenesulfonic acid (1,8-ANS) is a fluorescent dye that exhibits high affinity for the hydrophobic surfaces of proteins. It has an excitation maximum at 350 nm and an emission maximum of 520 nm when free in solution. However, when bound to proteins, such as intestinal fatty acid binding protein (FABP2), the emission maxima shift to 468-477 nm, and the fluorescence intensity increases. This property makes 1,8-ANS well-suited for determining the affinity of hydrophobic ligands to their corresponding binding proteins.
Uses
Used in Biochemistry and Molecular Biology:
8-Anilino-1-naphthalenesulfonic acid is used as a fluorescent probe to identify hydrophobic sites on proteins. This allows researchers to study the binding of hydrophobic ligands, such as free fatty acids, to their corresponding binding proteins, such as FABPs. The dye's fluorescence properties change upon binding, providing a sensitive and specific method for detecting interactions between proteins and hydrophobic molecules.
Used in Analytical Chemistry:
8-Anilino-1-naphthalenesulfonic acid can be used as a fluorescent indicator in analytical chemistry to detect the presence of hydrophobic compounds in samples. Its fluorescence properties make it a useful tool for the qualitative and quantitative analysis of hydrophobic substances in various matrices.
Used in Drug Discovery and Development:
In the pharmaceutical industry, 8-Anilino-1-naphthalenesulfonic acid can be employed as a tool to study the binding interactions between drug candidates and their target proteins. This information can be valuable for optimizing the design of new drugs and understanding their mechanisms of action.
Used in Environmental Monitoring:
8-Anilino-1-naphthalenesulfonic acid can be utilized in environmental monitoring to detect the presence of hydrophobic pollutants, such as oil spills or organic contaminants, in water samples. Its sensitivity to hydrophobic surfaces makes it a useful tool for assessing the extent of contamination and tracking the spread of pollutants in the environment.
Check Digit Verification of cas no
The CAS Registry Mumber 82-76-8 includes 5 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 2 digits, 8 and 2 respectively; the second part has 2 digits, 7 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 82-76:
(4*8)+(3*2)+(2*7)+(1*6)=58
58 % 10 = 8
So 82-76-8 is a valid CAS Registry Number.
InChI:InChI=1/C16H13NO3S/c18-21(19,20)15-11-5-7-12-6-4-10-14(16(12)15)17-13-8-2-1-3-9-13/h1-11,17H,(H,18,19,20)/p-1
82-76-8Relevant articles and documents
Preparation method of N-phenyl-1- naphthylamino-8-sulfonic acid
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Paragraph 0037; 0038; 0039; 0040; 0041; 0042, (2016/10/27)
The invention discloses a preparation method of N-phenyl-1- naphthylamino-8-sulfonic acid, which comprises the following steps: 1. condensation: adding aniline into a pot, starting stirring, adding concentrated sulfuric acid, adding 1-naphthylamino-8-sulfonic acid, dehydrating, and condensing at 155+/-2 DEG C; 2. neutralization: adding a soda ash solution to perform neutralization, transferring into a neutralization pot, and regulating the material of the material to 9-11; 3. vapor distillation: regulating the volume of the material with aniline water, adding vat powder to perform vapor distillation, and carrying out extraction when the aniline content in the solution is less than 3+/-0.5%, wherein the aniline content of 2-4% in the solution is used as the switch point; 4. decolorization: starting stirring, regulating the pH value of the liquid to 6.5-7.0, adding activated carbon, and heating to 94-95 DEG C; and 5. acid precipitation: adding dilute sulfuric acid until the residual acid content of the acid precipitation solution is 1.8-2.0%, cooling, discharging to obtain the N-phenyl-1- naphthylamino-8-sulfonic acid, and washing with 1.0-2.0% acid water twice.
