83-29-4Relevant articles and documents
Secobarbital artificial antigen and preparation method thereof
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Paragraph 0029; 0063-0065, (2018/09/28)
The objective of the invention is to provide a secobarbital artificial antigen and a preparation method thereof. According to the invention, malonate and urea are used as main raw materials for synthesis of the secobarbital antigen. The method comprises the following steps: reacting halogenated pentane with malonate to prepare 2-(1-methylbutyl)malonate; subjecting a malonate derivative and urea toa cyclization reaction to prepare barbitone derivative; reacting long-chain halogenated olefine acid with the barbitone derivative to prepare a hapten; and reacting the hapten reacts with N-hydroxysuccinimide and N,N-cyclohexylcarbodiimide under nitrogen protection to obtain active ester, subjecting the active ester and a protein to a coupling reaction, and simultaneously removing a trifluoroacetyl protecting group so as to obtain the secobarbital artificial antigen. The preparation method provided by the invention is simple in process, low in production cost, and not limited in raw materialsources. The prepared artificial antigen can be used for animal immunization to obtain corresponding antibodies, is used for the research of various barbitone immunoassays and the production of rapiddetection kits, and has a wide application scope.
Photochemical degradation of barbituric acid derivatives. Part 6: Products of photolysis of 5-allyl-5-(1-methylbutyl)barbituric acid-secobarbital
Barton,Bojarski
, p. 630 - 631 (2007/10/02)
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