856925-72-9

Basic information

• Product Name: DEOXYBLEBBISTATIN
• Synonyms: DEOXYBLEBBISTATIN;1,2,3,9-Tetrahydro-6-Methyl-1-phenyl-4H-pyrrolo[2,3-b]quinolin-4-one
• CAS NO: 856925-72-9
• Molecular Formula: C₁₈H₁₆N₂O
• Molecular Weight: 276.33
• Product Categories: All Inhibitors;Inhibitors
• Mol File: 856925-72-9.mol
• Article Data: 4

Chemical Properties

• Melting Point: 126-128°C
• Appearance: /
• Storage Temp.: -20°C Freezer
• Solubility: Chloroform (Slightly), Ethyl Acetate (Slightly)
• CAS DataBase Reference: DEOXYBLEBBISTATIN(CAS DataBase Reference)
• NIST Chemistry Reference: DEOXYBLEBBISTATIN(856925-72-9)
• EPA Substance Registry System: DEOXYBLEBBISTATIN(856925-72-9)

Safety Data

• Hazardous Substances Data: 856925-72-9(Hazardous Substances Data)

Usage

Chemical Properties

Brown Solid

Upstream product

• 4641-57-0 1-phenylpyrrolidin-2-one 
• 18595-16-9 2-amino-5-methylbenzoic acid methyl ester 
• 2941-78-8 2-Amino-5-methylbenzoic acid 
• 591-50-4 iodobenzene 
• 1177356-70-5 (3aS)-3a-hydroxy-6-methyl-1-phenyl-1,2,3,3a-tetrahydro-4H-pyrrolo[2,3-b]quinolin-4-one 

Downstream Products

• 1177356-70-5 (±)-1,2,3,3a-tetrahydro-3a-hydroxy-6-methyl-1-phenyl-4Hpyrrolo[2,3-b]quinolin-4-one 
• 1177356-70-5 (3aR)-3a-hydroxy-6-methyl-1-phenyl-1,2,3,3a-tetrahydro-4H-pyrrolo[2,3-b]quinolin-4-one 
• 1177356-70-5 (3aS)-3a-hydroxy-6-methyl-1-phenyl-1,2,3,3a-tetrahydro-4H-pyrrolo[2,3-b]quinolin-4-one 

Relevant articles and documents

Dynamics of Oxygen-Independent Photocleavage of Blebbistatin as a One-Photon Blue or Two-Photon Near-Infrared Light-Gated Hydroxyl Radical Photocage

Development of versatile, chemically tunable photocages for photoactivated chemotherapy (PACT) represents an excellent opportunity to address the technical drawbacks of conventional photodynamic therapy (PDT) whose oxygen-dependent nature renders it inadequate in certain therapy contexts such as hypoxic tumors. As an alternative to PDT, oxygen free mechanisms to generate cytotoxic reactive oxygen species (ROS) by visible light cleavable photocages are in demand. Here, we report the detailed mechanisms by which the small molecule blebbistatin acts as a one-photon blue light-gated or two-photon near-infrared light-gated photocage to directly release a hydroxyl radical (?OH) in the absence of oxygen. By using femtosecond transient absorption spectroscopy and chemoselective ROS fluorescent probes, we analyze the dynamics and fate of blebbistatin during photolysis under blue light. Water-dependent photochemistry reveals a critical process of water-assisted protonation and excited state intramolecular proton transfer (ESIPT) that drives the formation of short-lived intermediates, which surprisingly culminates in the release of ?OH but not superoxide or singlet oxygen from blebbistatin. CASPT2//CASSCF calculations confirm that hydrogen bonding between water and blebbistatin underpins this process. We further determine that blue light enables blebbistatin to induce mitochondria-dependent apoptosis, an attribute conducive to PACT development. Our work demonstrates blebbistatin as a controllable photocage for ?OH generation and provides insight into the potential development of novel PACT agents.

Improved synthesis and comparative analysis of the tool properties of new and existing D-ring modified (S)-blebbistatin analogs

(S)-Blebbistatin is a widely used research tool to study myosin II, an important regulator of many motility based diseases. Its potency is too low to be of clinical relevance, but identification of analogs with enhanced potency could deliver leads for tar