86088-83-7 Usage
Description
Fibronectin, a glycoprotein found in the skin's basal layer, is an important component that plays a significant role in maintaining a healthy basal layer. It is produced by many types of cells and constitutes 1 to 3 percent of the total cellular protein of fibroblasts. Fibronectin functions to strengthen the attachments among collagen and elastin fibers, fibroblasts, and other cells in the dermis with connective tissues. Its presence may also be important to cell growth, and there is some evidence that it may act as a cell regenerator by reversing abnormal cell formation and growth.
Uses
Used in Cosmetics Industry:
Fibronectin is used as a humectant and moisturizing agent for skin creams and lotions, providing a surface protective effect and maintaining skin hydration.
Used in Biotechnology and Cell Culture:
Fibronectin solution from human fibroblasts is used for various applications, such as purification of human extracellular matrix, coating of tissue culture plates with extracellular matrix components, and during cell culture. It is particularly useful for xeno-free, feeder-free culture, where cells are passaged into human fibronectin along with other components.
Fibronectin from human plasma can be used with various cell types, including epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, and endothelial cells. It is recommended for use as a cell culture substratum at concentrations ranging from 1-5 μg/cm2 or 0.5-50 μg/mL, depending on the cell type and research objectives.
General Description:
Native fibronectin purified from human plasma is an effective agent for promoting attachment of cells to commonly-used culture substrates.
Biochem/physiol Actions
Fibronectin is a high molecular mass extracellular matrix glycoprotein involved in many cellular processes, including tissue repair, embryogenesis, blood clotting, cell migration/adhesion, and embryogenesis and development. It is used in vitro as a substrate to enhance adherence and proliferation of many cell types. It has the capacity to bind to macromolecules such as collagen, fibrin, glycosaminoglycans, and Clq, apart from some bacteria.
Purification Methods
This glycoprotein contains 5-12% of carbohydrate. It has been purified by glycine fractionation and DEAE-cellulose chromatography. This material is dissolved in 0.25M Tris-phosphate buffer pH 7.0, diluted to 20% and glycine added gradually till 2.1M when the temperature falls to below 15o. The precipitate contains mainly fibrinogen. The supernatant is discarded, and the precipitate is treated with an equal volume of H2O, cooled (to 0o) and precipitated by adding EtOH to 16% (v/v) at -4o. The precipitate contains some CI (Cold Insoluble) globulin, fibronectin and small quantities of other proteins. To remove these the precipitate is dissolved in 0.25M Tris-phosphate buffer (pH 7.0) ca 0.5% and purified by DEAE-cellulose chromatography after diluting the buffer to 0.05M buffer. [Morrison et al. J Am Chem Soc 70 3103 1948, Mosesson & Umfleet J Biol Chem 245 5728 1970, Mosesson & Amrani Blood 56 145 1980, Akiyama & Yamada Adv Enzymol 59 51 1987.]
Check Digit Verification of cas no
The CAS Registry Mumber 86088-83-7 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 8,6,0,8 and 8 respectively; the second part has 2 digits, 8 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 86088-83:
(7*8)+(6*6)+(5*0)+(4*8)+(3*8)+(2*8)+(1*3)=167
167 % 10 = 7
So 86088-83-7 is a valid CAS Registry Number.
