9001-12-1 Usage
Description
Collagenase is a class of enzymes that can break down native collagen, the primary fibrous component of animal extracellular connective tissue. Produced by certain bacteria, such as Clostridium, and also generated by the animal body as part of its immune response, collagenases target connective tissue in muscle cells and other organs. They cleave peptide bonds within collagen and facilitate the creation of collagen by breaking down pro-collagen, collagen's precursor, once it is secreted by the cell. Bacterial collagenases have a broader substrate specificity and can degrade both water-insoluble native collagens and water-soluble denatured ones, unlike animal collagenases that only split collagen in its native triple-helical conformation. Collagenases have a molecular weight ranging from 68 kilodaltons (kDa) to 130 kDa, with an optimal pH for stability between 6.3 and 7.5 and a theoretical isoelectric point of 5.62. Various compounds and molecules can act as activators or inhibitors of collagenase activity, with Ca2+ and Zn2+ ions being known activators and EDTA, EGTA, cysteine, histidine, DTT, 2-mercaptoethanol, o-phenanthroline, Hg2+, Pb2+, Cd2+, Cu2+, and Zn2+ being known inhibitors.
Uses
Used in Tissue Culture and Cell Isolation:
Collagenase is used as a tissue digesting enzyme for liberating individual cells from connective components in tissue samples. It is particularly useful in cartilage dispersal at concentrations of 1-2 mg/ml, although species-specific and tissue-specific concentrations should be determined through literature searches.
Used in Research:
Collagenase is utilized in the investigation of collagen's structure and biosynthesis, as well as in the dispersion of cells for tissue culture studies.
Used in Medical Treatments:
Collagenase, Type I, Clostridium histolyticum is used as a powder and solvent injection kit for the treatment of Dupuytren's contracture, a condition where the connective tissue in the palm becomes thickened and causes the fingers to curl.
References
1.https://en.wikipedia.org/wiki/Collagenase
2.http://www.wisegeek.com/what-is-collagenase.htm
3.http://www.rockland-inc.com/collagenase.aspx
4.http://www.worthington-biochem.com/cls/default.html
Biochem/physiol Actions
Effective release of cells from tissue requires the action of collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca2+) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA)4; β-mercaptoethanol; glutathione, reduced; thioglycolic acid, sodium; and 2,2′-dipyridyl; 8-hydroxyquinoline are known to inhibit the enzyme activity.
Purification Methods
Collagenase is purified by using N-ethylmaleimide to activate the enzyme, and wheat germ agglutinin-agarose affinity chromatography [Callaway et al. Biochemistry 25 4757 1986].
Check Digit Verification of cas no
The CAS Registry Mumber 9001-12-1 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 9,0,0 and 1 respectively; the second part has 2 digits, 1 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 9001-12:
(6*9)+(5*0)+(4*0)+(3*1)+(2*1)+(1*2)=61
61 % 10 = 1
So 9001-12-1 is a valid CAS Registry Number.