9002-13-5

Basic information

• Product Name: UREASE
• Synonyms: jackbeanurease;UREASE, SP-OP;UREASE AMIDOHYDROLASE;UREA AMIDOHYDROLASE;UREA AMIDOLYASE;UREA AMYDOLYASE;UREASE;UREASE, FROM JACK BEAN
• CAS NO: 9002-13-5
• Molecular Weight: 30.06900
• EINECS: 232-656-0
• Product Categories: Biochemistry;Enzyme;Hydrolase;Metabolomics;3.5.x.x Acting on C-N other than peptides;Diagnostic and Analytical EnzymesMetabolomics;General Metabolic Enzymes I-ZEnzyme Class Index;3.x.x.x Hydrolases;Application Index;Enzymes, Inhibitors, and Substrates;General Metabolic Enzymes;Diagnostic and Analytical EnzymesResearch Essentials;EnzymesMetabolomics;Core Bioreagents
• Mol File: 9002-13-5.mol
• Article Data: 0

Chemical Properties

• Appearance: yellow/powder
• Storage Temp.: 2-8°C
• Water Solubility: It is slightly soluble in water.
• Merck: 14,9870
• CAS DataBase Reference: UREASE(CAS DataBase Reference)
• NIST Chemistry Reference: UREASE(9002-13-5)
• EPA Substance Registry System: UREASE(9002-13-5)

Safety Data

• Hazard Codes: Xn
• Statements: 36/37/38-42
• Safety Statements: 22-24-26-36/37-45-36-23
• WGK Germany: 1
• RTECS: YU1700000
• F: 10-21
• TSCA: Yes
• Hazardous Substances Data: 9002-13-5(Hazardous Substances Data)

Usage

Description

Urease is an enzyme found in the natural environment, including water and soil, as well as in the human body, where it is associated with protein degradation. It is capable of urea hydrolysis, resulting in the production of ammonia and carbamate, which can lead to an increase in pH. Urease and the ammonia generated during urea hydrolysis may be toxic to human tissue and could play a role in long-lasting diseases such as atherosclerosis, urinary tract infections, or rheumatoid arthritis.

Uses

Used in Clinical Applications:
Urease is used as a clinical reagent for the determination of urea in body fluids.
Used in Environmental Analysis:
Urease from Canavalia ensiformis is used for urea determination in various samples, such as legumes, and may be useful for the detection of pathogens as well as heavy-metal ions.
Used in Agricultural Applications:
Urease, an enzyme, converts urea into ammonium carbonate [(NH4)2CO3] that releases ammonia, activating the hydrolysis of urea. This process can affect crop growth and soil health.
Used in Environmental Wastewater Treatment:
Urease can be used to analyze urea concentration in blood, urine, alcoholic beverages, natural water, and environmental wastewaters, as well as to analyze heavy metal content in natural waters, wastewaters, and soil.
Used in Special Cases:
Urease can be used to remove urea from artificial kidney dialyzates, alcohol beverages, and fertilizer wastewaters, control or shift pH for multi-enzyme reaction systems, hydrolyze urea as sources of ammonia or carbon dioxide, and for wastewater reclamation for life support systems in space.

References

[1] Iwona Konieczna, Paulina ?arnowiec, Marek Kwinkowski, Beata Kolesińska, Justyna Fr?czyk, Zbigniew Kamiński, Wies?aw Kaca (2012) Bacterial Urease and its Role in Long-Lasting Human Diseases, Curr Protein Petp Sci., 13, 789-806 [2] Yingjie Qin, Joaquim M. S. Cabral (2002) Review: Properties and Applications of Urease, Biocatalysis and Biotransformation, 20, 1-14

Biochem/physiol Actions

Urease catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is involved in nitrogen metabolism and urea degradation. Urease from Canavalia ensiformis binds 2 nickel ions per subunit .