9014-01-1 Usage
Description
EC 3.4.21.14, also known as protease from Bacillus licheniformis, is a serine endoproteinase with a broad specificity towards native and denatured proteins. It is active under alkaline conditions and is isolated from the fermentation of Bacillus licheniformis. This proteolytic enzyme is known for its catalytic, non-catalytic, and ancillary domains and is classified as an endopeptidase and serine protease.
Uses
Used in Total Dietary Fiber Assays:
EC 3.4.21.14 is used as a proteolytic enzyme in Total Dietary Fiber Assays (TDF-100A) for the digestion of proteins.
Used in Neurolemma Digestion in Moths:
In the antennal lobe of the moth Heliothis virescens, EC 3.4.21.14 is used as a protease to digest the neurolemma.
Used in Determining Proteolytic Activity:
EC 3.4.21.14 from Bacillus sp. is used to determine its proteolytic activity by the spectrophotometric method.
Defination
Proteases (EC 3.4.21.62) belong to the class of enzymes known as hydrolases, which catalyze hydrolysis of various bonds in presence of water. Proteases are also referred to as Peptidases or Proteinases. Proteases catalyze proteolysis of peptide bonds in polypeptides, proteins and selective hydrolysis of carboxylic esters and amino esters. There are different classes of Proteases, i.e. serine, threonine, cysteine, aspartate, glutamic acid and metallo – proteases.
Biochem/physiol Actions
Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate. Proteases, secreted from Bacillus sp., typically have molecular weights ranging from 20,000 to 30,000. They are typcially stabilized by Ca2+ and have high isoelectric points.
Safety Profile
Moderately toxic by ingestion. Aneye irritant. When heated to decomposition it emits toxicfumes of NOx.
Purification Methods
This alkaline protease is purified 211-fold by affinity chromatography using 4-(4-aminophenylazo)phenylarsonic acid complex to activated CH-Sepharose 4B. It is inhibited by 2-phenylethane boronic acid, PMSF, 3,4-dichloroisocoumarin, acetone and benzamide. [Chandraskaren & Dhar Anal Biochem 150 141 1985, Schomburg & Schomburg Springer Handbook of Enzymes 2nd Edn vol 7 p 286 2002.] Synexin (from bovine liver) M 47,000 Da. This Ca binding protein is purified by (NH4)2SO4 precipitation, then by a specific pH step elution from a chromatofocusing medium in the absence of ampholytes. The pI is 7.5. [Scott et al. Anal Biochem 149 163 1985.]
Check Digit Verification of cas no
The CAS Registry Mumber 9014-01-1 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 9,0,1 and 4 respectively; the second part has 2 digits, 0 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 9014-01:
(6*9)+(5*0)+(4*1)+(3*4)+(2*0)+(1*1)=71
71 % 10 = 1
So 9014-01-1 is a valid CAS Registry Number.