9064-47-5

Basic information

• Product Name: HISTONE TYPE VIII-S
• Synonyms: histone type iii-S from calf thymus;histone type iii-ss from calf thymus;histone type viii-S from calf thymus;Histones nucleo froM calf thyMus;Histones,dried(Calf thyMus);Histone III (calfthyMus) (9CI);f3histonefraction;histoneiii(calfthymus)
• CAS NO: 9064-47-5
• Molecular Formula: NULL
• Molecular Weight: 0
• EINECS: 297-235-6
• Product Categories: Protein Kinase C (PKC)Peptides for Cell Biology;Protein Kinase Related Peptides;Protein Kinase Substrates;Serine/Threonine Kinase Biology;Serine/Threonine Kinase Substrates;Biochemicals and Reagents;Bovine;Proteins and Derivatives
• Mol File: 9064-47-5.mol
• Article Data: 0

Chemical Properties

• Appearance: /
• Storage Temp.: 2-8°C
• Solubility: H2O: soluble10mg/mL, clear to hazy, colorless to light yellow
• BRN: 1895465
• CAS DataBase Reference: HISTONE TYPE VIII-S(CAS DataBase Reference)
• NIST Chemistry Reference: HISTONE TYPE VIII-S(9064-47-5)
• EPA Substance Registry System: HISTONE TYPE VIII-S(9064-47-5)

Safety Data

• WGK Germany: 3
• RTECS: MS3450700
• F: 10-21
• Hazardous Substances Data: 9064-47-5(Hazardous Substances Data)

Usage

Description

Histone Type VIII-S is a variant of histone proteins that play a crucial role in the packaging and organization of DNA within the cell nucleus. It is part of the nucleosome structure, which consists of an octameric core of histone proteins, including H3, H4, H2A, and H2B, around which DNA is wound. The core histone proteins have globular domains and are composed of basic amino acids at their N-terminal ends. Histone H2A has an additional amino acid at its C-terminal end, forming a histone tail. The nucleosome structure is further stabilized by linker histones during chromatin condensation.

Uses

Used in Biochemical Research:
Histone Type VIII-S is used as a positive control in various in vitro kinase assays, such as the Cdc2-cyclin B1 assay, to examine the ability of the enzyme to phosphorylate GST-CD-4 protein. This application helps researchers study the phosphorylation events and their impact on cellular processes.
Used in Enzyme Activity Assays:
Histone Type VIII-S serves as a substrate in Cdk5 activity assays, allowing researchers to evaluate the activity of the Cdk5 enzyme, which is involved in various cellular functions, including neuronal development and cell cycle regulation.
Used in Histone H1 Kinase Assays:
This histone variant is utilized in histone H1 kinase assays, providing a means to assess the activity of kinases that specifically target histone H1, an important component in chromatin structure and function.
Used in Poly(ADP-ribose) (PAR) Polymerase Activity Assessment:
Histone Type VIII-S acts as a PAR acceptor in the evaluation of in vitro PAR polymerase activity. PAR polymerase enzymes play a role in DNA repair and genomic stability, and their activity can be measured using this histone variant as a substrate.
Used in Protein Kinase Activity Measurement:
As an acceptor of 32P from [γ-32P]ATP, Histone Type VIII-S enables the measurement of protein kinase activity in lysates from small and large luteal cells. This application is valuable for understanding the role of protein kinases in cellular signaling and regulation.

Biochem/physiol Actions

Histones are a group of proteins characterized by high levels of lysine and arginine that form reversible complexes with DNA. Based on the fractions, the molar mass of histones varies between 11-21kDa. Five different fractions have been isolated and characterized. They include H1 (lysine rich), H2a and H2b that are slightly lysine rich, H3 and H4 that are arginine rich. H1 acts as a link between "beads" (nu bodies) on the chromatin chain.