9068-38-6

Basic information

• Product Name: REVERSE TRANSCRIPTASE, M-MLV, RNASEH+
• Synonyms: M-MLV REVERSE TRANSCRIPTASE;MOLONEY MURINE LEUKEMIA VIRUS REVERSE TRANSCRIPTASE;Nucleotidyltransferase, deoxyribonucleate, RNA-dependent;REVERSE TRANSCRIPTASE, FOR MOL. BIOLOGY, DRY ICE, 500 U*;REVERSE TRANSCRIPTASE FROM AVIAN*MYELOBL ASTOSIS VIR;CYSCRIBE REVERSE TRANSCRIPTASE;AMV REVERSE TRANSCRIPTASE, REC., INDUSTRIAL GMP GRADE;amv-reverse transcriptase from avian myeloblastosis virus
• CAS NO: 9068-38-6
• Molecular Weight: 0
• EINECS: 232-964-5
• Product Categories: 2.7.x.x Phosphorus containing groupsMore...Close...;AmplificationMolecular Biology;cDNA LabelingApplication Index;PCRPlant Molecular Biology;RT-PCREnzyme Class Index;2.x.x.x Transferases;Functional Profiling of Stem Cells;Gene Expression&Analysis;Molecular Biology Tested;Nucleic Acid Amplification;PCR/Amplification;RT-PCRMolecular Biology;Stem Cell Biology;Enzyme
• Mol File: 9068-38-6.mol
• Article Data: 0

Chemical Properties

• Appearance: colorless/
• Storage Temp.: −70°C
• CAS DataBase Reference: REVERSE TRANSCRIPTASE, M-MLV, RNASEH+(CAS DataBase Reference)
• NIST Chemistry Reference: REVERSE TRANSCRIPTASE, M-MLV, RNASEH+(9068-38-6)
• EPA Substance Registry System: REVERSE TRANSCRIPTASE, M-MLV, RNASEH+(9068-38-6)

Safety Data

• WGK Germany: 3
• Hazardous Substances Data: 9068-38-6(Hazardous Substances Data)

Usage

Description

REVERSE TRANSCRIPTASE, M-MLV, RNASEH+ is a group of enzymes that play a crucial role in the synthesis of complementary DNA (cDNA) from RNA templates. M-MLV Reverse Transcriptase is an enzyme derived from the Moloney Murine Leukemia Virus, which is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. It is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand. RNASEH+ is an enzyme possessed by retroviruses that allows transcription of DNA from an RNA template. The discovery of this enzyme challenged the widely held central dogma, which stated that the information flow in nature was always from DNA to RNA.

Uses

Used in Molecular Biology Research:
REVERSE TRANSCRIPTASE, M-MLV, RNASEH+ is used as a tool for first-strand cDNA synthesis, which is essential for various molecular biology applications, such as gene expression analysis, cloning, and functional studies.
Used in cDNA Library Preparation:
REVERSE TRANSCRIPTASE, M-MLV, RNASEH+ is used as an enzyme for the preparation of cDNA libraries, which are crucial for genome research and the identification of novel genes.
Used in RT-PCR Assays:
REVERSE TRANSCRIPTASE, M-MLV, RNASEH+ is used as a component in 2-step RT-PCR assays, allowing for the amplification and detection of specific RNA transcripts.
Used in Cloning:
REVERSE TRANSCRIPTASE, M-MLV, RNASEH+ is used as an enzyme for the synthesis of cDNA that is further used in cloning, enabling the production of recombinant proteins and the study of gene function.
Used in Dideoxy DNA Sequencing:
REVERSE TRANSCRIPTASE, M-MLV, RNASEH+ is used as an enzyme in dideoxy DNA sequencing, a technique used to determine the nucleotide sequence of a DNA molecule.
Used in Primer Extension and RNA Sequencing:
REVERSE TRANSCRIPTASE, M-MLV, RNASEH+ is used as an enzyme for primer extension and RNA sequencing, allowing for the analysis of RNA structure and function.
Used in 3'-end Labeling of DNA Fragments:
REVERSE TRANSCRIPTASE, M-MLV, RNASEH+ is used as an enzyme for the 3'-end labeling of DNA fragments, which is useful for various molecular biology techniques, such as DNA fingerprinting and the study of DNA-protein interactions.
Used in Generation of Single-stranded Probes:
REVERSE TRANSCRIPTASE, M-MLV, RNASEH+ is used as an enzyme for the generation of single-stranded probes for genomic footprint experiments, which help in understanding the regulation of gene expression and the binding of transcription factors to DNA.
Used in Diagnostics and Therapeutics Development:
REVERSE TRANSCRIPTASE, M-MLV, RNASEH+ is used as a tool in the development of diagnostic tests and therapeutics targeting RNA viruses, such as HIV and SARS-CoV-2, by enabling the study of viral RNA and the development of antiviral drugs and vaccines.

Purification Methods

This enzyme produces the complementary DNA from the RNA (as template). These are purified by solubilising the virus with non-ionic detergents. Lysed virions are adsorbed on DEAE-cellulose or DEAE-Sephadex columns, and the enzymes are eluted with a salt gradient, then chromatographed on a phosphocellulose column and fractions with enzyme activity are eluted in a salt gradient. They are also purified from other viral proteins by affinity chromatography on a pyran-Sepharose column. [Verna Biochim Biophys Acta 473 1 1977, Smith Methods Enzymol 65 560 1980; see commercial catalogues for other transcriptases.]