91809-67-5 Usage
Description
6-Carboxytetramethylrhodamine is a fluorescent dye that is widely utilized for the covalent labeling of oligonucleotides in DNA analysis. It exhibits excitation and emission maxima at 543 nm and 572 nm, respectively, making it a valuable tool in various scientific applications.
Uses
Used in DNA Analysis:
6-Carboxytetramethylrhodamine is used as a fluorescent label for oligonucleotides, enabling the detection and analysis of DNA sequences. Its distinct excitation and emission maxima facilitate the study of DNA-protein interactions and contribute to the effectiveness of DNA FRET experiments.
Used in DNA-Protein Binding Studies:
In the field of molecular biology, 6-Carboxytetramethylrhodamine serves as a fluorescent probe for investigating the binding of proteins to DNA. Its properties allow researchers to monitor and analyze the interactions between DNA and proteins, providing insights into the mechanisms of gene regulation and other biological processes.
Used in DNA FRET Experiments:
6-Carboxytetramethylrhodamine is employed as a donor or acceptor dye in F?rster Resonance Energy Transfer (FRET) experiments, which are crucial for studying the dynamics and conformational changes of DNA molecules. Its fluorescence properties make it suitable for detecting changes in the proximity of DNA and other biomolecules during FRET processes.
Used as a Reporter or Quencher Dye in RT-PCR:
In the context of reverse transcription polymerase chain reaction (RT-PCR), 6-Carboxytetramethylrhodamine is utilized as a standard reporter or quencher dye. Its fluorescence characteristics are instrumental in monitoring the amplification of DNA during the RT-PCR process, ensuring accurate and reliable results.
Used in the Free Acid Form of 6-Carboxytetramethylrhodamine Single Isomer:
The free acid form of 6-carboxytetramethylrhodamine single isomer is a specific variant of the dye that can be used in various applications, such as the development of fluorescent probes or the synthesis of other fluorescent compounds. This form offers versatility and specificity for researchers working with fluorescent dyes in different scientific and industrial applications.
Check Digit Verification of cas no
The CAS Registry Mumber 91809-67-5 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 9,1,8,0 and 9 respectively; the second part has 2 digits, 6 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 91809-67:
(7*9)+(6*1)+(5*8)+(4*0)+(3*9)+(2*6)+(1*7)=155
155 % 10 = 5
So 91809-67-5 is a valid CAS Registry Number.
InChI:InChI=1/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)20-11-14(24(28)29)5-8-17(20)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)
