92-31-9 Usage
Description
Basic Blue 17 is a synthetic dye that belongs to the azo dyes family. It is characterized by its blue color and is soluble in water. Basic Blue 17 is known for its ability to bind to proteins and other macromolecules, making it useful in various applications.
Uses
Used in Hemostatic Applications:
Basic Blue 17 is used as a hemostatic agent to control bleeding and promote blood clotting. Its ability to bind to proteins and other macromolecules helps in stabilizing the blood clot and preventing further bleeding.
Used in Histology:
Basic Blue 17 is used as a metachromatic dye in a wide variety of histological staining procedures. Its unique staining properties allow for the differentiation and visualization of various cellular structures and tissues.
Used in Textile Industry:
Basic Blue 17 is used as a direct dye for dyeing and printing of wool and silk fabrics. Its affinity for proteins and other macromolecules in the fibers allows for a strong and long-lasting color.
Used as a Biological Stain:
Basic Blue 17 is also used as a biological stain to visualize and differentiate various biological samples, such as bacteria, fungi, and other microorganisms. Its staining properties help in the identification and classification of these organisms.
Originator
Blutene,Abbott,US,1953
Preparation
by heavy chromate salt in the presence of sodium content and deal with N1,N1-dimethylbenzene-1,4-diamine, transforming it into the content and sulfonic acid, this product then through heavy chromate salt and O-Methylaniline processing, into a unique of sulfur acid amine generation, then use heavy chromate salt and copper sulfate closed loop and oxidation, Finally,?the?zinc chloride?salt?precipitation
Manufacturing Process
As taken from US Patent 416,055 (probably the oldest patent on the
manufacture of a currently-used drug): In carrying out this process about 6
pbw of dimethyl-p-phenylenediamine was dissolved in about 18 pbw of
hydrochloric acid of about 1.16 specific gravity and then a solution of about
3.8 pbw of nitrite of soda in about 6 pbw of water was gradually added. The
hydrochlorate of nitroso-dimethylaniline thus produced in the well-known
manner is then submitted to the reducing action of zinc-dust by adding, first
about 30 pbw of hydrochloric acid of about 1.16 specific gravity and then (in
small portions at a time) about 10 pbw of zinc-dust as is well understood by
chemists. The solution of hydrochlorate of paramido-dimethylaniline thus
obtained is afterwards diluted with about 250 pbw of water and then the
uncombined hydrochloric acid contained in the solution is, if any, neutralized
by the addition of an alkali. There are then added about 16 pbw of sulfate of
alumina and about 13 pbw of thiosulfate of sodium, (hyposulfite of soda) and
immediately afterwards a solution of about 5 pbw of bichromate of potash in
about 60 pbw of water is quickly run in.
In this stage of the process the formation of an acid sulfureted compound of
paramidodimethylaniline takes place, possessing the formula
C8H11N2SSO3H(paramido-dimethylaniline-thiosulfonic acid). Without previous
separation of this intermediate compound a solution of about 5.3 pbw of
orthotoluidine, in the requisite amount of dilute hydrochloric acid (about 6
pbw of hydrochloric acid, SG about 1.16, diluted with about 6 pbw water) and
shortly afterwards a solution of about 14 pbw of bichromate of potash in
about 160 parts by weight of water is then added under constant agitation,
when a precipitate will be formed chiefly consisting of a green indamine
possessing in its dry condition the formula C15H17N3S2O3. In order to
transform the same into toluidine-blue, about 50 pbw of a solution of chloride
of zinc of about 1.5 specific gravity are added and the mixture thus obtained
is boiled during about half an hour, when, after cooling, the toluidine-blue thus
formed will separate out and may then be filtered and purified, if necessary,
by repeated solution in water and precipitation by means of chloride of sodium
and chloride of zinc.
In the above described process the sulfate of alumina may be dispensed with
and replaced by as much hydrochloric, sulfuric, or acetic acid as will be
required to liberate the thiosulfuric acid from the thiosulfate of sodium
employed.
Toluidine-blue prepared as above described presents the following
characteristic properties: It consists principally of the hydrochlorate of dimethyltoluthionine, the composition of which corresponds to the formula
C15H15N3SHCl.
Standard( Cotton )
Light Fastness
Fading
Stain
C
2-3
Check Digit Verification of cas no
The CAS Registry Mumber 92-31-9 includes 5 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 2 digits, 9 and 2 respectively; the second part has 2 digits, 3 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 92-31:
(4*9)+(3*2)+(2*3)+(1*1)=49
49 % 10 = 9
So 92-31-9 is a valid CAS Registry Number.
InChI:InChI=1/C15H16N3S.ClH/c1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H/q+1;/p-1
92-31-9Relevant articles and documents
TOLUIDINE BLUE O DRUG SUBSTANCE AND USE THEREOF FOR IN VIVO STAINING AND CHEMOTHERAPEUTIC TREATMENT OF DYSPLASTIC TISSUES
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Page 17-19, (2008/06/13)
The invention comprises an improved process for preparing TBO drug products includes the steps: (1) synthesising an indamine; (2) converting said indamine into an S-indaminyl thiosulfate; and (3) adding an oxidizing catalyst agent, complexing agent, and an acid to said S-indaminyl thiosulfate to formulate TBO and C-4-methyl regioisomer, and derivatives thereof. The invention further comprises new compositions of matter that are useful for detecting dysplastic tissue, as well as, treating dysplastic tissue, namely, TBO products predominantly comprised of peaks eight, peak six, or a combination thereof. N,N-dimenthyl-p-phenylenediamine as a starting material results in a TBO product composition comprised of peaks eight, seven, six, and five in the approximate ratios 33:5:5:1, respectively. Whereas N-dimethyl-p-phenylenediamine as a starting material results in a TBO demetylated product composition comprised of peaks six, five, tree and two in the approximate ratio 33:5:5:1, respectively. The invention further comprises an improved PLC method for analyzing the improved TBO drug product, the improvement comprising the addition of an ion-pairing reagent in a first mobile phase and forming a second mobile phase composition comprising 5o% alcohol by volume.
