93384-17-9

Basic information

• Product Name: PBR322 PLASMID DNA
• Synonyms: PBR322 DNA;PBR322 VECTOR;PBR325 PLASMID DNA;PBR322 PLASMID DNA;PLASMID PBR322 DEOXYRIBONUCLEIC ACID;PLASMID PBR322 DNA;DNA, PBR322;DEOXYRIBONUCLEIC ACID, PLASMID (PBR 322 PLASMID)
• CAS NO: 93384-17-9
• Molecular Weight: 0
• EINECS: 297-227-2
• Mol File: 93384-17-9.mol
• Article Data: 0

Chemical Properties

• Appearance: /lyophilized powder
• Storage Temp.: 2-8°C
• CAS DataBase Reference: PBR322 PLASMID DNA(CAS DataBase Reference)
• NIST Chemistry Reference: PBR322 PLASMID DNA(93384-17-9)
• EPA Substance Registry System: PBR322 PLASMID DNA(93384-17-9)

Safety Data

• WGK Germany: 3
• F: 3-10-21
• Hazardous Substances Data: 93384-17-9(Hazardous Substances Data)

Usage

Description

PBR322 Plasmid DNA is a multipurpose cloning vector originally constructed for use in Escherichia coli. It serves as a versatile tool in molecular biology and genetic engineering, facilitating various applications such as cloning, selection, and expression of recombinant molecules, construction of shuttle vectors, and vectors for nucleotide sequencing. Additionally, it aids in studying elements involved in gene expression and serves as a model system for understanding prokaryotic plasmid replication.

Uses

Used in Genetic Engineering and Molecular Biology Research:
PBR322 Plasmid DNA is used as a cloning vector for manipulating and studying genes of interest. It enables researchers to insert foreign DNA fragments into the plasmid, inactivate specific genes, and analyze gene expression and regulation.
Used in Recombinant DNA Technology:
PBR322 Plasmid DNA is used as a tool for recombinant DNA technology, allowing for the creation of genetically modified organisms with desired traits. The Pst I and Pvu I cleavage sites in the ampicillin gene, or the Cla I, Hind III, BamH I, and Sal I sites in the tetracycline gene, provide convenient points for the insertion of foreign DNA fragments.
Used in Shuttle Vector Construction:
PBR322 Plasmid DNA is used as a basis for constructing shuttle vectors, which are capable of replicating in both prokaryotic and eukaryotic systems. This allows for the transfer and expression of genetic material between different organisms.
Used in Nucleotide Sequencing:
PBR322 Plasmid DNA is used as a vector for nucleotide sequencing, enabling the determination of the order of nucleotides within a DNA molecule and providing valuable information about gene structure and function.
Used in Studies of Gene Expression Elements:
PBR322 Plasmid DNA is used to study elements involved in gene expression, such as promoters, terminators, and regulatory regions, which are crucial for understanding how genes are controlled and expressed within cells.
Used in Plasmid DNA Standardization:
PBR322 Plasmid DNA is used as a standard for comparing and quantifying plasmid DNA in various experiments, ensuring accuracy and consistency in molecular biology research.
Used in Artificial Metallonuclease Activity Studies:
PBR322 Plasmid DNA has been utilized in studies of artificial metallonuclease activity, where the plasmid is subjected to specific enzymes to investigate their effects on DNA structure and function.
Used in Ferrous Ion-Induced Strand Breaks Research:
PBR322 Plasmid DNA from E. coli RR1 has been employed to study the mechanism of ferrous ion-induced strand breaks, which are mediated through hydrogen peroxide. This research contributes to understanding the effects of metal ions on DNA integrity and potential implications for genetic stability and disease.

Biochem/physiol Actions

This plasmid is derived from the ColE1-type plasmid pMB1 and shares the same type of replication mechanism and controls as ColE1 and relatives.