95047-71-5Relevant articles and documents
Synthesis of New Cationic Schiff Base Complexes of Copper(II) and Their Selective Binding with DNA
Tanaka, Tomohide,Tsurutani, Keiko,Komatsu, Atsushi,Ito, Toyofumi,Iida, Kazumi,Fujii, Yuki,Nakano, Yoshiharu,Usui, Yoshiharu,Fukuda, Yutaka,Chikira, Makoto
, p. 615 - 629 (2007/10/03)
A series of cationic salen-type Schiff base complexes of copper(II), the general formula of which is [Cu{R3N+-CH2-C6H3(O -)-CH=N-}2X]Br2 (R: CH3, C2H5, C3H7, C4H9; X: C2H4, C3H6, C6H10, C6H4, C10H6), was newly prepared, and the molecular structure of complex 1 (R=CH3, X=C2H4) was determined by an X-ray crystal structure analysis. The binding mode and binding constants (Kb) of all the complexes with calf thymus DNA were investigated at an ionic strength of I = 0.05 (NaCl + HEPES buffer, pH = 7.2) using induced CD and the UV-vis spectra. Those complexes with aliphatic X group selectively bound to the groove of DNA, and the Kb's were in the range of 102 - 103 mol dm-3 (site size base pairs n = 7.2 - 7.4). However, those with aromatic X group selectivity intercalated to the base pairs, and the Kb's were in the range of 104 - 105 mol dm-3 (n = 2.8 - 4.0). These binding modes were confirmed based on the salt dependence of Kb's. In addition, 1 and 10 (R = CH3, X = C6H4) were found to exhibit AT-sequence affinity from the induced CD spectra and Kb's for poly(dA-dT)-poly(dA-dT), poly(dG-dC)-poly(dG-dC), and poly(dA-dC)-poly(dT-dG).
